GP.MOT: A novel glycophorin variant identified in a Japanese blood donor. Issue 10 (9th August 2021)

• Record Type: Journal Article
• Authors: Oda, Akira; Suzuki, Yumi; Isa, Kazumi; Ogasawara, Kenichi; Yabe, Ryuichi; Kimura, Takafumi; Uchikawa, Makoto; Tsuno, Nelson Hirokazu
• Is Part Of: Transfusion. Volume 61:Issue 10(2021)
• Page Start: 2825
• Page End: 2829
• Publication Date: 2021-08-09
• Subjects: glycophorin A (GPA) -- glycophorin B (GPB) -- glycophorin variant -- MNS blood groups; Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.16535
• Languages: English
• ISSNs: 0041-1132

Abstract

Abstract: Background: In this study, we identified a novel glycophorin variant (GP.MOT) in a Mi a ‐positive Japanese blood donor. The proband with this glycophorin variant was discovered by antigen screening of samples from 475, 493 Japanese blood donors using monoclonal anti‐Mi a . Study design and methods: Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti‐GPA, anti‐MUT or anti‐Mur. Genome DNA was extracted from whole blood, and the GYPA / GYPB was analyzed by polymerase chain reactions and Sanger sequencing. Results: The MNS blood group of the proband was M + N + w S‐s + with the presence of other low‐frequency antigens including Mi a, Mur, MUT, and KIPP. A 43‐kDa molecule, which is almost equivalent in size to glycophorin A (GPA), was identified by immunoblotting using monoclonal anti‐MUT and anti‐Mur. Sanger sequencing clearly indicated that the proband had two different GYPA*M alleles at SNP rs62334651 ( GYPA*M232 + 55A and GYPA*M232 + 55G ), as well as a GYP ( B‐A ) hybrid allele ( GYP*MOT ) with breakpoints located on pseudoexon 3 of GYPB from c.210 to c.219. Discussion: We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20–45)‐GP Ψ B (46–70)‐GPA (71–149), which has not been previously reported.