Adipose-Derived Stem Cell Exosomes Inhibit Hypertrophic Scaring Formation by Regulating Th17/Treg Cell Balance. (12th October 2022)

• Record Type: Journal Article
• Authors: Wang, Hongtao; Li, Yan; Yue, Zhang; Liu, Yang; Chen, Qiaohua; Hu, Dahai; Han, Juntao
• Other Names: Mishra Sandip K Academic Editor.
• Is Part Of: BioMed research international. Volume 2022(2022)
• Publication Date: 2022-10-12
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2022/9899135
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : Aiming to reveal the role of ADCS-Exos in secretion of inflammatory factors, Th17 and regulatory T (Treg) cell differentiation from naïve CD4+ T cells in hypertrophic scaring formation and maturation is explored. ELISA, qRT-PCR, and immunoblotting are performed to assay the local inflammatory factors IL-6, IL-10, IL-17A, and TNF- α, and transcriptional factors of ROR ϒ t and Foxp3, in scaring tissue from patients and mice wound models treated with or without ADCS-Exos. Immunohistochemistry staining and immunoblotting are conducted to assay the extracellular matrix (ECM) deposition in vitro and in vivo. The results show that IL-6, IL-10, IL-17A, TNF- α, ROR ϒ t, and Foxp3 are increased on mRNA and protein levels in hypertrophic scaring compared with atrophic scaring and normal skin. Naïve CD4 + T cells treated with ADCS-Exos in vitro can produce significantly less IL-6, IL-17A, TNF- α, and ROR ϒ t and more IL-10 and Foxp3 on mRNA and protein levels. In addition, mice in ADSC-Exos-treated group demonstrate less collagen deposition; decreased IL-17A, TNF- α, and ROR ϒ t; and increased IL-10 and Foxp3 production.