Biocompatible scaffolds constructed by chondroitin sulfate microspheres conjugated 3D-printed frameworks for bone repair. (1st January 2023)

• Record Type: Journal Article
• Authors: Guo, Liangyu; Chen, Hao; Li, Yuanli; Zhou, Juan; Chen, Jinghua
• Is Part Of: Carbohydrate polymers. Volume 299(2023)
• Publication Date: 2023-01-01
• Subjects: Gel-MA modified gelatin -- nHAP nano-hydroxyapatite -- HAP hydroxyapatite -- CSA chondroitin sulfate A -- MC3T3-E1 Mouse embryo osteoblast precursor cells -- LAP Lithium Phenyl (2, 4, 6-trimethylbenzoyl) phosphinate -- G10 bio-ink prepared by using 10 wt% Gel-MA, 21 wt% HAP and 0.2 wt% LAP -- G12 bio-ink prepared by using 12 wt% Gel-MA, 28 wt% HAP and 0.24 wt% LAP -- G10-F external frame prepared by using G10 -- G12-F external frame prepared by using G12 -- Mc microspheres prepared by using 8 wt% Gel-MA, 5 wt% CSA and 0.16 wt% LAP -- M microspheres prepared by using 8 wt% Gel-MA and 0.16 wt% LAP -- G10-F@Mc composite scaffold prepared by filling Mc into the pores of the G10-F and photo-crosslinked -- G12-F@Mc composite scaffold prepared by filling Mc into the pores of the G12-F and photo-crosslinked -- G10-F@M composite scaffold prepared by filling M into the pores of the G10-F and photo-crosslinked -- G10-F@Mc@Cell MC3T3-E1 loaded scaffold prepared by loading MC3T3-E1 on the G10-F@Mc -- ALP alkaline phosphatase -- ARS alizarin red -- Runx 2 runt-related transcription factor 2 -- OCN osteocalcin -- Col 1 collagen 1 -- BMD bone mineral density -- Conn.D connectivity density -- BV/TV bone volume/total volume -- Tb.sp trabecular separation -- OPG osteoprotegerin -- TRAP tartrate-resistant acid phosphatase -- RANKL nuclear factor kB receptor activator of NF KB ligand -- IL-1β interleukin 1β; Composite scaffold -- 3D printing -- Microsphere -- Chondroitin sulfate -- Bone regeneration; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120188
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Most bone repair scaffolds are multi-connected channel structure, but the hollow structure is not conducive to the transmission of active factors, cells and so on. Here, microspheres were covalently integrated into 3D-printed frameworks to form composite scaffolds for bone repair. The frameworks composed of double bond modified gelatin (Gel-MA) and nano-hydroxyapatite (nHAP) provided strong support for related cells climbing and growth. Microspheres, which were made of Gel-MA and chondroitin sulfate A (CSA), were able to connect the frameworks like bridges, providing channels for cells migration. Additionally, CSA released from microspheres promoted the migration of osteoblasts and enhanced osteogenesis. The composite scaffolds could effectively repair mouse skull defect and improve MC3T3-E1 osteogenic differentiation. These observations confirm the bridging effect of microspheres rich in chondroitin sulfate and also determine that the composite scaffold can be as a promising candidate for enhanced bone repair.