A mutation in SlCHLH encoding a magnesium chelatase H subunit is involved in the formation of yellow stigma in tomato (Solanum lycopersicum L.). (December 2022)

• Record Type: Journal Article
• Authors: Li, Yushun; Wang, Xinyu; Zhang, Qinghua; Shen, Yuanbo; Wang, Jin; Qi, Shiming; Zhao, Pan; Muhammad, Tayeb; Islam, Md. Monirul; Zhan, Xiangqiang; Liang, Yan
• Is Part Of: Plant science. Volume 325(2022)
• Publication Date: 2022-12
• Subjects: Tomato -- Yellow stigma -- Map-based clone -- Mg-chelatase H subunit -- Chlorophyll biosynthesis; ys yellow stigma -- CHLH magnesium chelatase H subunit -- EMS ethylmethylsulfone -- QTL quantitative trait locus -- WT wild type -- AC Alisa Craig -- ABA abscisic acid -- BSA bulked segregant analysis -- SNP single nucleotide polymorphisms -- CAPS cleaved amplified polymorphism sequence -- dCAPS derived cleaved amplified polymorphism sequence -- InDel insertions/deletions of DNA sequences -- RNAi RNA interference -- HEMA glutamyl t-RNA reductase -- HEMG protoporphyrin oxidase -- CHLM magnesium chelatase methyltransferase -- CAO chlorophyllide a oxygenase -- HEMF coproporphyrinogen III oxidase -- CHLI magnesium chelatase I subunit -- CHLD magnesium chelatase D subunit -- GUN genome uncoupling -- Chs chlorophyll synthase -- SD standard deviation; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111466
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Chlorophylls are ubiquitous pigments responsible for the green color in plants. Changes in the chlorophyll content have a significant impact on photosynthesis, plant growth and development. In this study, we used a yellow stigma mutant ( ys ) generated from a green stigma tomato WT by using ethylmethylsulfone (EMS)-induced mutagenesis. Compared with WT, the stigma of ys shows low chlorophyll content and impaired chloroplast ultrastructure. Through map-based cloning, the ys gene is localized to a 100 kb region on chromosome 4 between dCAPS596 and dCAPS606. Gene expression analysis and nonsynonymous SNP determination identified the Solyc04g015750, as the potential candidate gene, which encodes a magnesium chelatase H subunit (CHLH). In ys mutant, a single base C to T substitution in the SlCHLH gene results in the conversion of Serine into Leucine (Ser92Leu) at the N-terminal region. The functional complementation test shows that the SlCHLH from WT can rescue the green stigma phenotype of ys . In contrast, knockdown of SlCHLH in green stigma tomato AC, observed the yellow stigma phenotype at the stigma development stage. Overexpression of the mutant gene Slys in green stigma tomato AC results in the light green stigma. These results indicate that the mutation of the N-terminal S92 to Leu in SlCHLH is the main reason for the formation of the yellow stigma phenotype. Characterization of the ys mutant enriches the current knowledge of the tomato chlorophyll mutant library and provides a novel and effective tool for understanding the function of CHLH in tomato. Highlights: SlCHLH controlling the stigma color formation and the S92 of N-terminal affects magnesium chelatase activity.