Hyaluronic acid hydrolysis using vacuum ultraviolet TiO2 photocatalysis combined with an oxygen nanobubble system. (1st January 2023)

• Record Type: Journal Article
• Authors: Cho, Sung Yeon; Lee, Seokjae; Yang, Kyungjik; Kim, Young Min; Choe, Deokyeong; Kim, Jeong Un; Lee, Dong-Un; Park, Jiyong; Roh, Young Hoon
• Is Part Of: Carbohydrate polymers. Volume 299(2023)
• Publication Date: 2023-01-01
• Subjects: Hyaluronic acid hydrolysis -- Nanobubble -- UV-TiO2 photocatalysis -- Endotoxin -- Antioxidant activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120178
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Advanced technologies for producing high-quality low molecular weight hyaluronic acid (LMW-HA) are required from the perspective of cost-efficiency and biosafety. Here, we report a new LMW-HA production system from high molecular weight HA (HMW-HA) using vacuum ultraviolet TiO2 photocatalysis with an oxygen nanobubble system (VUV-TP-NB). The VUV-TP-NB treatment for 3 h resulted in a satisfactory LMW-HA (approximately 50 kDa measured by GPC) yield with a low endotoxin level. Further, there were no inherent structural changes in the LMW-HA during the oxidative degradation process. Compared with conventional acid and enzyme hydrolysis methods, VUV-TP-NB showed similar degradation degree with viscosity though reduced process time by at least 8-fold. In terms of endotoxin and antioxidant effects, degradation using VUV-TP-NB demonstrated the lowest endotoxin level (0.21 EU/mL) and highest radical scavenging activity. This nanobubble-based photocatalysis system can thus be used to produce biosafe LMW-HA cost-effectively for food, medical, and cosmetics applications. Graphical abstract: Unlabelled Image