Enhanced biomass and lipid production by light exposure with mixed culture of Rhodotorula glutinis and Chlorella vulgaris using acetate as sole carbon source. (November 2022)

• Record Type: Journal Article
• Authors: Gong, Guiping; Wu, Bo; Liu, Linpei; Li, Jianting; He, Mingxiong; Hu, Guoquan
• Is Part Of: Bioresource technology. Volume 364(2022)
• Publication Date: 2022-11
• Subjects: Red yeast -- Microalgae -- Microbial consortia -- Acetate utilization; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128139
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Acetate was applied to produce microbial biomass and lipids by yeast/algae co-culture. Co-culture facilitates acetate utilization efficiency thus reducing culture period. Increasing algae proportion promoted biomass productivity and acetate assimilation. Light-exposed synergetic effect further increased cell growth and lipid titer. More neutral lipids and linolenic acid were accumulated with light-exposed co-culture. Abstract: Microbial biomass and lipid production with mixed-culture of Rhodotorula glutinis and Chlorella vulgaris using acetate as sole carbon source was investigated. Synergistic effect of mixed-culture using 20 g/L acetate significantly promoted cell growth and acetate utilization efficiency. Increasing the proportion of algae in co-culture was beneficial for biomass and lipid accumulation and the optimal ratio of yeast/algae was 1:2. Light exposure further enhanced biomass and lipid titer with 6.9 g/L biomass and 2.6 g/L lipid (38.3 % lipid content) obtained in a 5L bioreactor. The results of lipid classes and fatty acid profiles moreover indicated that more neutral lipids and linolenic acid were synthesized in mixed-culture under light exposure condition, suggesting the great potential in applications of biofuels production. This study provided new insight and strategy for economical microbial biomass and lipid production by light-exposed mixed-culture using inexpensive acetate as carbon source.