Inhibitory effect of infliximab on orthodontic tooth movement in male rats. (December 2022)

• Record Type: Journal Article
• Authors: Fu, Chaoran; Gan, Lei; Yang, Benyi; Wu, Yilin; Meng, Bowen; Wu, Dongle; Zheng, Jinxuan; Cao, Yang
• Is Part Of: Archives of oral biology. Volume 144(2022)
• Publication Date: 2022-12
• Subjects: TNF-α tumour necrosis factor-α -- RANKL receptor activator of nuclear factor κB ligand -- RANK receptor activator of nuclear factor κB -- TRAP Tartrate-resistant acid phosphatase -- OPG osteoprotegerin; Infliximab -- Orthodontic tooth movement -- Osteoclast -- Tumour necrosis factor-α; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2022.105573
• Languages: English
• ISSNs: 0003-9969

Abstract

Abstract: Objective: This study aimed to investigate the effect of infliximab on orthodontic tooth movement in rats. Materials and methods: Sixty male Sprague-Dawley rats were randomly divided into two groups: saline group and infliximab group. The two groups of rats received weekly intraperitoneally injection of saline or infliximab (5 mg/kg), respectively. After four weeks of injection, five rats in each group were euthanized and orthodontic appliances were placed in the other twenty-five rats each. On days 1, 3, 7, 14 and 21, five rats from each group were euthanised. Maxillae of all the rats were collected and examined by micro-computed tomography, haematoxylin and eosin staining, tartrate-resistant acid phosphatase staining, and immunohistochemical staining of tumour necrosis factor (TNF)-α, receptor activator of nuclear factor κB ligand (RANKL), receptor activator of nuclear factor κB (RANK), and osteoprotegerin (OPG). All data were analysed with Mann-Whitney test. Results: Infliximab inhibited orthodontic tooth movement and decreased osteoclastogenesis on the compression side during orthodontic tooth movement. The elevated TNF­α level, induced by orthodontic force, was decreased by infliximab. Furthermore, infliximab reduced the expression of RANKL and RANK, while increased the expression of OPG on the compression side. Conclusion: Infliximab inhibits orthodontic tooth movement by reducing levels of TNF-α, RANKL, and RANK, while increasing level of OPG, and decreasing osteoclastogenesis on the compression side of periodontium. Highlights: Infliximab slows down orthodontic tooth movement in rats. Infliximab inhibits osteoclast formation in periodontium during tooth movement. Infliximab reduces TNF­α on the compression side of orthodontic tooth movement. Infliximab may function by inhibiting RANKL/RANK pathway.