Enhancement of the polyethylene terephthalate and mono-(2-hydroxyethyl) terephthalate degradation activity of Ideonella sakaiensis PETase by an electrostatic interaction-based strategy. (November 2022)

• Record Type: Journal Article
• Authors: Yin, Qingdian; You, Shengping; Zhang, Jiaxing; Qi, Wei; Su, Rongxin
• Is Part Of: Bioresource technology. Volume 364(2022)
• Publication Date: 2022-11
• Subjects: Polyethylene terephthalate (PET) -- IsPETase -- Electrostatic interaction -- PET degradation -- MHETase activity; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128026
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The electrostatic interaction-based strategy stabilized the structure of Is PETase. The Is PETase I139R variant exhibited 3.6-times higher PET degradation activity. The kcat of Is PETase S92K/D157E/R251A variant towards MHET showed 1.74-fold shift. The Is PETase S92K/D157E/R251A variant realized PET depolymerization to solely TPA. Abstract: The serious environmental pollution that came up with the continuously growing demand for polyethylene terephthalate (PET) has attracted global concern. The Is PETase which has shown the highest PET degradation activity under ambient temperature is a promising enzyme for PET biodegradation, while poor thermostability limited its practical application. Herein, an electrostatic interaction-based strategy was applied for rational design of Is PETase towards enhanced thermostability. The Is PETase I139R variant displayed the highest Tm value of 56.4 °C and 3.6-times higher PET degradation activity. Molecular simulations demonstrated that the introduction of salt bridges stabilized the local structures, resulting in robust thermostability. Meanwhile, the Is PETase S92K/D157E/R251A not only exhibited higher thermostability but also showed a 1.74-fold kcat increase towards mono-(2-hydroxyethyl) terephthalate, which ultimately achieved PET depolymerization to complete monomer TPA. Collectively, the electrostatic interaction-based strategy, together with the derived Is PETase variants, could help promote the bio-recycle of PET, reducing the severe global burden of PET waste.