Minimal residual disease detected by droplet digital PCR in peripheral blood stem cell grafts has a prognostic impact on high-risk neuroblastoma patients. Issue 10 (October 2022)

• Record Type: Journal Article
• Authors: Nino, Nanako; Ishida, Toshiaki; Nakatani, Naoko; Lin, Kyaw San; Win, Kaung Htet Nay; Mon, Cho Yee; Nishimura, Akihiro; Inoue, Shotaro; Tamura, Akihiro; Yamamoto, Nobuyuki; Uemura, Suguru; Saito, Atsuro; Mori, Takeshi; Hasegawa, Daiichiro; Kosaka, Yoshiyuki; Nozu, Kandai; Nishimura, Noriyuki
• Is Part Of: Heliyon. Volume 8:Issue 10(2022)
• Publication Date: 2022-10
• Subjects: Neuroblastoma (NB) -- Minimal residual disease (MRD) -- Peripheral blood stem cell (PBSC) -- Neuroblastoma-associated mRNAs (NB-mRNAs) -- Droplet digital PCR (ddPCR); Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2022.e10978
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstracts: More than half of high-risk neuroblastoma (NB) patients have experienced relapse due to the activation of chemoresistant minimal residual disease (MRD) even though they are treated by high-dose chemotherapy with autologous peripheral blood stem cell (PBSC) transplantation. Although MRD in high-risk NB patients can be evaluated by quantitative PCR with several sets of neuroblastoma-associated mRNAs (NB-mRNAs), the prognostic significance of MRD in PBSC grafts (PBSC-MRD) is unclear. In the present study, we collected 20 PBSC grafts from 20 high-risk NB patients and evaluated PBSC-MRD detected by droplet digital PCR (ddPCR) with 7NB-mRNAs (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNA). PBSC-MRD in 11 relapsed patients was significantly higher than that in 9 non-relapsed patients. Patients with a higher PBSC-MRD had a lower 3-year event-free survival (P = 0.0148). The present study suggests that PBSC-MRD detected by ddPCR with 7NB-mRNAs has a prognostic impact on high-risk NB patients. Abstract : Neuroblastoma (NB); Minimal residual disease (MRD); Peripheral blood stem cell (PBSC); Neuroblastoma-associated mRNAs (NB-mRNAs); Droplet digital PCR (ddPCR).