In vitro analysis of the effects of plant-derived chondroitin sulfate from intestinal barrier to chondrocytes. (November 2022)

• Record Type: Journal Article
• Authors: Galla, Rebecca; Ruga, Sara; Ferrari, Sara; Saccone, Sabrina; Saccuman, Laura; Invernizzi, Marco; Uberti, Francesca
• Is Part Of: Journal of functional foods. Volume 98(2022)
• Publication Date: 2022-11
• Subjects: Plant-derived chondroitin sulfate -- Intestinal absorption -- Chondrocytes -- Osteoarthritis; GAGs Glycosaminoglycans -- CS Chondroitin Sulfate -- C4S Chondroitin-4-Sulfate -- C6S Chondroitin-6-Sulfate -- OA Osteoarthritis -- TFPS Tremella Fuciformis -- SFDA Chinese Food and Drug Administration -- ACAN Aggrecan -- CD44 Cluster of Differentiation 44 -- OPG osteoprotegerin -- MMP-13 metalloproteinase-13 -- ROS Reactive Oxygen Species -- DMEM Dulbecco's Modified Eagle's Medium -- FBS foetal bovine serum -- TBE Tris/Borate/EDTA buffer -- DMMB 1, 9-Dimethylmethylene blue -- EMA European Medicinal Agency -- FDA Food Drug Amministration -- TEER Trans Epithelial Elettric Resistence -- TJ Tight Junction -- PG-4 Human Chondroitin sulfate proteoglycan 4 -- OCLN Human Occludin -- TJP1 Human Tight Junction Protein 1 -- NFKβ Nuclear factor-kβ -- ADAMTS-5 a disintegrin and metalloproteinase with thrombospondin motifs 5; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105285
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Greendroitine® is a new plant-derived chondroitin sulfate with low molecular weight. Greendroitine® is able to cross the intestinal barrier. Greendroitine® is able to exert beneficial effects on chondrocytes in vitro also in OA condition. Greendroitine® is a promising new nutraceutical candidate to the oral intake in human. Abstract: Chondroitin is well known to regulate several biological functions and to exert a critical role in the regulation of joint physiology maintaining proper chondrocyte activity. The aim of this study was to explore the ability of a new chondroitin, from plant origin, to induce different biological effects analyzing the link between certain chemical properties and the specific biological significance. Its biological effects were analyzed by intestinal permeability and in vitro osteoarthritis conditions verifing data to other chondroitin forms from animal and non-animal sources. Plant-derived chondroitin shows a heterogeneous chemical-biological profile, which may be responsible for many specific functions observed in vitro . Furthermore, it exhibits a better absorption profile without affecting the intestinal barrier and modulates disintegrin and metalloproteinase with thrombospondin 5 motifs to prevent cartilage degradation. Plant-derived chondroitin, Greendroitin®, can be considered an excellent new strategy to improve cartilage trophism, based on surprising biological properties in osteoarthritis in vitro model.