Excellent anammox performance driven by stable partial denitrification when encountering seasonal decreasing temperature. (November 2022)

• Record Type: Journal Article
• Authors: Liu, Qiyu; Peng, Yongzhen; Zhao, Yang; Zhao, Qi; Li, Xiyao; Zhang, Qiong; Sui, Jun; Wang, Chuanxin; Li, Jianwei
• Is Part Of: Bioresource technology. Volume 364(2022)
• Publication Date: 2022-11
• Subjects: Anammox -- Decreasing temperature -- Partial-denitrification -- Anoxic biofilm -- Biological nitrogen removal; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.128041
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Anammox contribution to nitrogen removal was robustly maintained at 91.3 ± 6.6 %. Compared with stable NO3 – → NO2 –, NO2 – → N2 was sensitive to temperature reduction. Anammox abundance was increased (0.56% to 1.22%) despite of decreased temperature. NO2 – accumulation at lower temperature was unexpectedly helpful to enrich anammox. Abstract: Effluent quality deterioration caused by seasonal temperature reductions in wastewater treatment systems using partial anammox technology is a challenge that cannot be ignored. Here, relationships of denitrification and anammox under decreasing temperature were investigated in an anoxic moving bed biofilm reactor (MBBR). Compared with stable partial-denitrification (NO3 – → NO2 – ), the NO2 – reduction to N2 was considerably inhibited when the temperature decreased, conversely helping to the competition of NO2 – for anammox. Namely, this transformation provided sufficient substrates for anammox bacteria. Although the TIN removal decreased slightly, anammox contribution was robustly maintained at 91.3 ± 6.6 %, even increased. High-throughput sequencing results revealed that anammox bacteria were enriched (0.56 % to 1.22 %). Moreover, qPCR results showed that increased ratio of hzsB /( nirK + nirS ) further supported anammox gained an enhancement. This study demonstrated partial-denitrification/anammox process using anoxic MBBR could maintain stable autotrophic nitrogen removal contribution when encountering temperature decrease, providing a new perspective on the application of mainstream anammox.