Paper-based sample processing for the fast and direct MS analysis of multiple analytes from serum samples. Issue 21 (28th September 2022)

• Record Type: Journal Article
• Authors: Zhang, Fu; Ji, Bin; Yan, Xiang-Hong; Lv, Shuang; Fang, Fang; Zhao, Shuang; Guo, Xiao-Lin; Wu, Zhi-Yong
• Is Part Of: Analyst. Volume 147:Issue 21(2022)
• Page Start: 4895
• Page End: 4902
• Publication Date: 2022-09-28
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d2an01261h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : The direct MS detection of amino acids obtained from serum was successfully demonstrated via a paper-based fast electrokinetic sample clean-up method. Abstract : Amino acids are closely related to human health, and their rapid determination is important for the rapid diagnosis, timely treatment, and assessment of serious diseases. In this work, we propose a novel paper-based sample-processing device combined with isotope-dilution MS for the fast analysis of 11 amino acids from blood samples. By using an isoelectric focusing electrokinetic separation method, without the aid of carrier ampholytes and the addition of inhibitors, this approach uses only the characteristic of the isoelectric point of the target amino acids to achieve separation and purification from other coexisting components in the medium; it can meet the requirements for mass spectrometry detection. Driven by a DC voltage, a stable and sharp pH gradient (pH 3–10.5 over 5 mm) can be established in a glass-fiber paper-based fluidic channel with a MS-friendly electrolyte. Amphoteric species can be well separated from the complex blood matrix and concentrated into a narrow band in the channel within 2 min, which is 20 times faster than a commercial kit method. The method can be applied to both liquid and dry spot samples, and the cleaned sample band can be simply dissolved for direct IDMS detection in ESI MRM mode. This method is a promising strategy for the rapid MS-based detection of amino acids from serum without pre-separation via liquid chromatography.