Sphingomonas cannabina sp. nov., isolated from Cannabis sativa L. 'Cheungsam'. Issue 10 (19th October 2022)

• Record Type: Journal Article
• Authors: Jeon, Doeun; Jiang, Lingmin; Peng, Yuxin; Seo, Jiyoon; Li, Zhun; Park, Seung-Hwan; Jeong, Rae-Dong; Park, Soon Ju; Jeong, Jae Cheol; Lee, Jiyoung
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 10(2022)
• Publication Date: 2022-10-19
• Subjects: novel species -- Sphingomonas -- Cannabis sativa -- endophytes -- average nucleotide identity; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005566
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-negative, aerobic, rod-shaped bacterium, designated DM2-R-LB4 T was isolated from Cannabis sativa L. 'Cheungsam' in Andong, Republic of Korea. The strain DM2-R-LB4 T grew at temperatures of 15–45 °C (optimum, 30–37 °C), pH of 5.5–9 (optimum, 8.0), and 0–2 % (w/v) NaCl concentration (optimum, 0%). Phylogenetic analyses based on the 16S rRNA gene sequences revealed that strain DM2-R-LB4 T is related to species of the genus Sphingomonas, and shared 97.8 and 97.5% similarity to Sphingomonas kyenggiensis KCTC 42244 T and Sphingomonas leidyi DSM 4733 T, respectively. The DNA G+C content was 67.9 mol% and genome analysis of the strain DM2-R-LB4 T revealed that the genome size was 4 386 171 bp and contained 4 009 predicted protein-coding genes. The average nucleotide identity (ANI) values between strain DM2-R-LB4 T and S. kyenggiensis KCTC 42244 T, and S. leidyi DSM 4733 T was 76.8 and 76.7 %, respectively, while the values of digital DNA–DNA hybridization (dDDH) were 20.7 and 20.6 %, respectively. C14 : 0 2-OH, C16 : 0, and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) were the major fatty acids (>10 %) in the strain DM2-R-LB4 T . The polar lipids comprised diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), sphingoglycolipid (SGL), glycolipid (GL), phospholipid (PL), and two unidentified polar lipids (L1 and L2). Ubiquinone-10 (Q-10) was the only respiratory quinone. The polyamine pattern was found to contain homospermidine, putrescine, and spermidine. The results of phylogenetic anlayses, polyphasic studies, revealed that strain DM2-R-LB4 T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas cannabina sp. nov., is proposed. The type strain is DM2-R-LB4 T (=KCTC 92075 T = GDMCC 1.3018 T ).