Hypoxia-induced carbonic anhydrase mediated dorsal horn neuron activation and induction of neuropathic pain. Issue 11 (29th November 2022)

• Record Type: Journal Article
• Authors: Da Vitoria Lobo, Marlene E.; Weir, Nick; Hardowar, Lydia; Al Ojaimi, Yara; Madden, Ryan; Gibson, Alex; Bestall, Samuel M.; Hirashima, Masanori; Schaffer, Chris B.; Donaldson, Lucy F.; Bates, David O.; Hulse, Richard Philip
• Is Part Of: Pain. Volume 163:Issue 11(2022)
• Page Start: 2264
• Page End: 2279
• Publication Date: 2022-11-29
• Subjects: Pain -- Neuron -- Endothelial -- Spinal cord -- Hypoxia -- Diabetes -- VEGF; Pain -- Periodicals; Douleur -- Périodiques; Anesthésie -- Périodiques; Pain; Electronic journals; Periodicals; Electronic journals; 616.0472
• DOI: 10.1097/j.pain.0000000000002627
• Languages: English
• ISSNs: 0304-3959

Abstract

Abstract : Supplemental Digital Content is Available in the Text. Reduced spinal cord blood flow leads to a hypoxia-mediated activation of hypoxia inducible factor 1α in dorsal horn neurons. This results in a carbonic anhydrase–dependent neuropathic pain. Abstract: Neuropathic pain, such as that seen in diabetes mellitus, results in part from central sensitisation in the dorsal horn. However, the mechanisms responsible for such sensitisation remain unclear. There is evidence that disturbances in the integrity of the spinal vascular network can be causative factors in the development of neuropathic pain. Here we show that reduced blood flow and vascularity of the dorsal horn leads to the onset of neuropathic pain. Using rodent models (type 1 diabetes and an inducible endothelial-specific vascular endothelial growth factor receptor 2 knockout mouse) that result in degeneration of the endothelium in the dorsal horn, we show that spinal cord vasculopathy results in nociceptive behavioural hypersensitivity. This also results in increased hypoxia in dorsal horn neurons, depicted by increased expression of hypoxia markers such as hypoxia inducible factor 1α, glucose transporter 3, and carbonic anhydrase 7. Furthermore, inducing hypoxia through intrathecal delivery of dimethyloxalylglycine leads to the activation of dorsal horn neurons as well as mechanical and thermal hypersensitivity. This shows that hypoxic signalling induced by reduced vascularity results in increased hypersensitivity and pain. Inhibition of carbonic anhydrase activity, through intraperitoneal injection of acetazolamide, inhibited hypoxia-induced pain behaviours. This investigation demonstrates that induction of a hypoxic microenvironment in the dorsal horn, as occurs in diabetes, is an integral process by which neurons are activated to initiate neuropathic pain states. This leads to the conjecture that reversing hypoxia by improving spinal cord microvascular blood flow could reverse or prevent neuropathic pain.