Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer. (22nd December 2020)

• Record Type: Journal Article
• Authors: Levink, Iris J. M.; Nesteruk, Kateryna; Visser, Dido I.; Sieuwerts, Anieta M.; Fernandes, Celio J.C.; Jansen, Maurice P.H.M.; van Driel, Lydi M.J.W.; Poley, Jan-Werner; Peppelenbosch, Maikel P.; Cahen, Djuna L.; Fuhler, Gwenny M.; Bruno, Marco J.
• Is Part Of: American journal of gastroenterology. Volume 115:Number 12(2020)
• Page Start: 2103
• Page End: 2108
• Publication Date: 2020-12-22
• Subjects: Stomach -- Diseases -- Periodicals; Intestines -- Diseases -- Periodicals; Gastroenterology -- Periodicals; Gastrointestinal Diseases -- Periodicals; Electronic journals; Periodicals; 616.33
• DOI: 10.14309/ajg.0000000000000939
• Languages: English
• ISSNs: 0002-9270

Abstract

Abstract : INTRODUCTION: Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers. METHODS: Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0–4 vs 4–8 vs 8–15 minutes). RESULTS: Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible. DISCUSSION: The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture.