3D bioprinting of in situ vascularized tissue engineered bone for repairing large segmental bone defects. (December 2022)

• Record Type: Journal Article
• Authors: Shen, Mingkui; Wang, Lulu; Gao, Yi; Feng, Li; Xu, Chuangye; Li, Sijing; Wang, Xiaohu; Wu, Yulan; Guo, Yao; Pei, Guoxian
• Is Part Of: Materials today bio. Volume 16(2022)
• Publication Date: 2022-12
• Subjects: 3D bioprinting -- In situ vascularization -- RNA sequencing Analysis -- Large segmental bone defects -- Tissue engineering; Alkaline phosphatase (ALP) -- Alizarin red S (ARS) -- analysis of variance (ANOVA) -- bone mesenchymal stem cells (BMSCs) -- bone mineral density (BMD) -- bone volume to tissue volume (BV/TV) -- complementary DNA (cDNA) -- 4′, 6-diamidino-2-phenylindole (DAPI) -- differentially expressed genes (DEGs) -- Dulbecco's modified Eagle's medium (DMEM) -- Dulbecco's phosphate-buffered saline (DPBS) -- ethylenediamine tetraacetic acid (EDTA) -- endothelial cells (ECs) -- extracellular matrix (ECM) -- fetal bovine serum (FBS) -- Fourier-transform infrared (FTIR) -- 3D printed GelMA hydrogel scaffold (G) -- 3D dual-extrusion bioprinted GelMA hydrogel and RAOECs-laden 3P hydrogel scaffold (G-3PR) -- 3D bioprinted BMSCs-laden GelMA hydrogel scaffold (GB) -- 3D dual-extrusion bioprinted BMSCs-laden GelMA hydrogel and RAOECs-laden 3P hydrogel scaffold (GB-3PR) -- gene ontology (GO) -- gelatin methacryloyl (GelMA) -- green fluorescent protein (GFP) -- glyceraldehyde-3-phosphate dehydrogenase (GAPDH) -- hematoxylin and eosin (H&E) -- lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate (LAP) -- micro-computed tomography (micro-CT) -- nuclear magnetic resonance (NMR) -- optical density (OD) -- paraformaldehyde (PFA) -- phosphate-buffered saline (PBS) -- polyethylene glycol (PEG) -- polylactic acid (PLA) -- PLA-PEG-PLA (3P) -- polyvinylidene fluoride (PVDF) -- radioimmunoprecipitation assay (RIPA) -- rat aortic endothelial cells (RAOECs) -- real-time polymerase chain reaction (RT-PCR) -- standard deviation (SD) -- tissue-engineered bone (TEB) -- tris buffered saline with Tween-20 (TBST); Materials science -- Periodicals; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 620.1
• DOI: 10.1016/j.mtbio.2022.100382
• Languages: English
• ISSNs: 2590-0064

Abstract

Abstract: Large bone defects remain an unsolved clinical challenge because of the lack of effective vascularization in newly formed bone tissue. 3D bioprinting is a fabrication technology with the potential to create vascularized bone grafts with biological activity for repairing bone defects. In this study, vascular endothelial cells laden with thermosensitive bio-ink were bioprinted in situ on the inner surfaces of interconnected tubular channels of bone mesenchymal stem cell-laden 3D-bioprinted scaffolds. Endothelial cells exhibited a more uniform distribution and greater seeding efficiency throughout the channels. In vitro, the in situ bioprinted endothelial cells can form a vascular network through proliferation and migration. The in situ vascularized tissue-engineered bone also resulted in a coupling effect between angiogenesis and osteogenesis. Moreover, RNA sequencing analysis revealed that the expression of genes related to osteogenesis and angiogenesis is upregulated in biological processes. The in vivo 3D-bioprinted in situ vascularized scaffolds exhibited excellent performance in promoting new bone formation in rat calvarial critical-sized defect models. Consequently, in situ vascularized tissue-engineered bones constructed using 3D bioprinting technology have a potential of being used as bone grafts for repairing large bone defects, with a possible clinical application in the future. Graphical abstract: Image 1 Highlights: 3D bioprinting was used to fabricate in situ vascularized tissue engineered bone. In situ bioprinted endothelial cells exhibited uniform distribution and greater seeding efficiency. 3D-bioprinted scaffold produced coupling between angiogenesis and osteogenesis.