Proteomic mapping and optogenetic manipulation of membrane contact sites. Issue 17 (16th September 2022)

• Record Type: Journal Article
• Authors: Lin, Gang; Shi, Wenyi; Zhang, Ningxia; Lee, Yi-Tsang; Wang, Youjun; Jing, Ji
• Is Part Of: Biochemical journal. Volume 479:Issue 17(2022)
• Page Start: 1857
• Page End: 1875
• Publication Date: 2022-09-16
• Subjects: inter-organelle communication -- membrane contact sites -- optogenetics -- proximity labelling; Biochemistry -- Periodicals; 572
• DOI: 10.1042/BCJ20220382
• Languages: English
• ISSNs: 0264-6021

Abstract

Abstract : Membrane contact sites (MCSs) mediate crucial physiological processes in eukaryotic cells, including ion signaling, lipid metabolism, and autophagy. Dysregulation of MCSs is closely related to various diseases, such as type 2 diabetes mellitus (T2DM), neurodegenerative diseases, and cancers. Visualization, proteomic mapping and manipulation of MCSs may help the dissection of the physiology and pathology MCSs. Recent technical advances have enabled better understanding of the dynamics and functions of MCSs. Here we present a summary of currently known functions of MCSs, with a focus on optical approaches to visualize and manipulate MCSs, as well as proteomic mapping within MCSs.