Age and composition of the thrombus retrieved by mechanical thrombectomy from patients with acute ischemic stroke are associated with revascularization and clinical outcomes. Issue 219 (November 2022)

• Record Type: Journal Article
• Authors: Shimizu, Hisao; Hatakeyama, Kinta; Saito, Kozue; Shobatake, Ryogo; Takahashi, Nobuyuki; Deguchi, Jun; Tokunaga, Hidemori; Shimada, Keiji; Nakagawa, Ichiro; Myochin, Kaoru; Sakai, Kazuya; Kubo, Masayuki; Yamashita, Atsushi; Obayashi, Chiho; Sugie, Kazuma; Matsumoto, Masanori
• Is Part Of: Thrombosis research. Issue 219(2022)
• Page Start: 60
• Page End: 69
• Publication Date: 2022-11
• Subjects: CE cardioembolism -- tPA tissue-type plasminogen activator -- ICA internal carotid artery -- MCA middle cerebral artery -- PCA posterior cerebral artery -- mRS modified Rankin Scale -- NIHSS National Institutes of Health Stroke Scale -- TOAST Trial of Org 10172 in Acute Stroke Treatment -- TICI thrombolysis in cerebral infarction -- RBCs red blood cells -- VWF von Willebrand factor; Thrombus age -- Mechanical thrombectomy -- Acute ischemic stroke -- Revascularization; Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2022.09.004
• Languages: English
• ISSNs: 0049-3848

Abstract

Abstract: Introduction: Understanding the composition of stroke thrombi retrieved by mechanical thrombectomy is essential to clarify the pathogenesis of stroke. However, it is difficult to evaluate thrombus composition precisely and objectively. Immunohistochemical staining was used to evaluate thrombus composition and age. Materials and methods: Consecutive thrombi ( n = 108) retrieved from patients who underwent mechanical thrombectomy for acute large-vessel ischemic stroke were retrospectively analyzed. Lytic features of granulocytes and CD163 were estimated as indicators of the age of the cardioembolic (CE) thrombus. Results: The stroke subtypes were as follows: CE, 74 cases; large artery atherosclerosis, 11; undetermined etiology, 12; and other determined etiology, 11. There were no statistical differences in thrombi composition according to stroke subtypes. The fibrin area was positively correlated with the red blood cell (RBC) and platelet areas. The following analysis was performed using CE only. Regarding age, the thrombus was judged as fresh in 30.0 % and older in 70.0 % based on the lytic features. The RBC areas of older thrombi were smaller than those of fresh thrombi. The puncture-to-reperfusion time of older thrombi was longer than that of fresh thrombi. Platelet-rich thrombi were associated with a greater number of maneuvers, a smaller prevalence of TICI 3, and unfavorable functional outcomes compared to platelet-poor thrombi. The number of CD163 positive cells in thrombi with anticoagulants was higher than in those without anticoagulants. Conclusion: Thrombus composition correlated with revascularization and clinical outcomes. The composition of an acute ischemic thrombus may reflect the pathophysiology of stroke and influence treatment efficacy. Graphical abstract: Unlabelled Image