Deep eutectic systems for carbonic anhydrase extraction from microalgae biomass to improve carbon dioxide solubilization. (November 2022)

• Record Type: Journal Article
• Authors: Craveiro, Rita; Dusschooten, Fleur; Nabais, Ana R.; Boboescu, Iulian; Lo, Calvin; Neves, Luísa A.; Sá, Marta
• Is Part Of: Journal of CO₂ utilization. Volume 65(2022)
• Publication Date: 2022-11
• Subjects: Tisochrysis lutea -- Chlorella vulgaris -- Spirulina sp. -- Carbonic Anhydrase -- Deep Eutectic System -- CO2 capture and utilization; Carbon dioxide -- Periodicals; Carbon dioxide -- Environmental aspects -- Periodicals; Carbon dioxide mitigation -- Periodicals; Carbon dioxide; Carbon dioxide -- Environmental aspects; Carbon dioxide mitigation; Periodicals; 628.53205
• DOI: 10.1016/j.jcou.2022.102225
• Languages: English
• ISSNs: 2212-9820

Abstract

Abstract: This work is the first proof-of-concept of the use of carbonic anhydrase (CA) enzyme from microalgae biomass, extracted with deep eutectic systems (DES), with the goal of engineering a solution that will lead to a breakthrough in the Carbon Capture and Utilization (CCU) strategy. Three distinct microalgae were processed – Tisochrysis lutea, Chlorella vulgaris, and Spirulina sp . – with three DES – Choline chloride-Urea (ChCl-U), Choline chloride-Poly(ethylene glycol) (ChCl-PEG), and Poly(ethylene glycol)-Urea (PEG-U). To evaluate the most promising microalgae-DES, CA activity was evaluated with a specific enzymatic activity kit and through CO2 solubility assays. Preliminary results indicate that: DES is a suitable solvent medium for CA extraction from microalgal biomass, preserving its activity (specific CA activity up to 0.70 mU.mg −1 ); CA extraction efficiency differs between DES and microalgal species, indicating the potential for further research; from the tested DES, the ones containing PEG were favorable to maintain CA activity (CO2 solubility up to 4 g CO2.g −1 DES). This work paves the way towards a disruptive CCU approach. Highlights: Carbonic anhydrase (CA) from microalgae was extracted with deep eutectic systems (DES). CA activity was evaluated by specific enzymatic activity kit and CO2 solubility assays. DES is a suitable solvent for preserving CA activity. CA extraction efficiency differs between DES and microalgal species. DES containing PEG were more favorable to maintain CA activity.