Rejuvenation of photoaged aged mouse skin using high-intensity focused ultrasound. Issue 10 (October 2022)

• Record Type: Journal Article
• Authors: Lee, Tiffany J.; Kim, Donggeon; Kim, Taehyun; Pak, Changsik John; Suh, Hyunsuk Peter; Hong, Joon Pio
• Is Part Of: Journal of plastic, reconstructive & aesthetic surgery. Volume 75:Issue 10(2022)
• Page Start: 3859
• Page End: 3868
• Publication Date: 2022-10
• Subjects: High-intensity focused ultrasound (HIFU) -- Photo-aging skin -- Skin rejuvenation; Surgery, Plastic -- Great Britain -- Periodicals; Reconstructive Surgical Procedures -- Periodicals; Surgery, Plastic -- Great Britain -- Periodicals; 617.9505
• DOI: 10.1016/j.bjps.2022.06.073
• Languages: English
• ISSNs: 1748-6815

Abstract

Summary: Background: High-intensity focused ultrasound (HIFU) therapy has emerged as an option for skin rejuvenation. However, the application against photo-damaged skin remains obscure. This study evaluates the effect of HIFU against photoaged skin using a mouse model. Methods: A total of 60 mice were used and divided into 3 groups; group 1: natural aging control group ( n = 20), group 2: UVB irradiation group ( n = 20), and group 3: UVB irradiation followed by HIFU treatment ( n = 20). The evaluation was made grossly by analyzing wrinkles and histologically by performing H&E, Toluidine Blue, Masson's Trichrome, and immunohistochemistry for TGF-β and MMP3. Imaging software was used to quantify the findings. Results: Gross findings showed HIFU treated group 3 with similar findings with the control group supporting the rejuvenation effect for photo-aged skin. Histology findings with H&E show a significant reduction in skin thickness after HIFU treatment (60.115 units (group 2) vs. 40.853 units (group 3), p <0.05). Toluidine Blue and Masson's Trichrome showed improved collagen array and significantly increased distribution for group 3 over group 2 (272, 879.88 units (group 2) vs. 533, 805.78 units (group 3), p <0.05). Immunohistochemistry for TGF-β showed a significantly higher value for group 3 (2.45450 units) over group 2 (0.58880 units) and MMP3 with a significantly lower value for group 3 (99, 180 units) over group 2 (559, 830 units) ( p <0.05). Conclusions: The treatment of HIFU supports the rejuvenation effect for photoaged skin. Findings show that HIFU provides benefits of collagen formation and rearrangement by enhancing TGF-β and inhibiting MMP3 activity. This study is the first animal study to show the direct effect of HIFU on photo-aged skin further supporting the use of HIFU in aging skin aiming to reverse the morphological effects of aging.