Arsenic biotransformation genes and As transportation in soil-rice system affected by iron-oxidizing strain (Ochrobactrum sp.). (1st December 2022)

• Record Type: Journal Article
• Authors: Xue, Shengguo; He, Xuan; Jiang, Xingxing; Pan, Weisong; Li, Waichin; Xia, Libing; Wu, Chuan
• Is Part Of: Environmental pollution. Volume 314(2022)
• Publication Date: 2022-12-01
• Subjects: Soil-rice system -- Iron oxidizing strain -- Ferrous iron oxidation -- Arsenic biotransformation gene; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.120311
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Arsenic (As) biotransformation in soil affects As biogeochemical cycling and is associated with As accumulation in rice. After inoculation with 1% iron-oxidizing bacteria (FeOB) in paddy soil, As speciation, As biotransformation genes in soil, As/Fe in Fe plaques, and As accumulation in rice were characterized. Compared with the control, the available As concentrations in soils decreased while amorphous and poorly crystalline Fe–Al oxidized As and crystalline Fe–Al oxidized As fractions increased of F (FeOB) and RF (rice and FeOB) treatments. Fe concentrations increased and positively correlated with As concentrations in Fe plaques on the rice root surface (***P < 0.001). Compared with R (rice), Monomethyl As (MMA), dimethyl As (DMA), arsenate (As(V)), and arsenite (As(III)) concentrations in rice plants showed a downwards trend of RF treatment. The As concentration in grains was below the National Standard for Food Safety (GB 2762-2017). A total of 16 As biotransformation genes in rhizosphere soils of different treatments (CK, F, R and RF were quantified by high-throughput qPCR (HT-qPCR). Compared with the control, the As(V) reduction and As transport genes abundance in other treatments increased respectively by 54.54%–69.17% and 54.63%–73.71%; the As(III) oxidation and As (de) methylation genes did not change significantly; however, several As(III) oxidation genes (aoxA, aoxB, aoxS, and arsH) increased. These results revealed that FeOB could reduce, transport As, and maybe also oxidize As. In addition, As(III) oxidation gene (aoxC) in rhizosphere soil was more abundant than in non-rhizosphere soil. It indicated that radial oxygen loss (ROL) promoted As(III) oxidation in rhizosphere soils. The results provide evidence for As biotransformation by ROL and FeOB in soil-rice system. ROL affects As oxidation and immobilization, and FeOB affects As reduction, transportation and may also affect As oxidation. Graphical abstract: Image 1 Highlights: FeOB increased the proportion of crystalline Fe–Al oxidized As in soil. FeOB decreased As availability and accumulation in soil-rice system. As biotransformation genes were quantified by HT-qPCR. Several As(III) oxidation genes (aoxA, aoxB, aoxS and arsH) abundance increased with FeOB. As(V) reduction and As transport genes abundance in soil-rice system increased with FeOB.