Isolation and characterisation of Pulsatilla Radix-utilising bacteria Pediococcus pentosaceus PR-1 from human faeces. Issue 1 (7th September 2022)

• Record Type: Journal Article
• Authors: Liu, Yue; Sun, Xiaoxia; Zhang, Jincan; Gao, Feng; Yu, Leilei; Dong, Lina; Zhang, Gangli; Wu, Changxin
• Is Part Of: FEMS microbiology letters. Volume 369:Issue 1(2022)
• Publication Date: 2022-09-07
• Subjects: Pulsatilla Radix -- Pediococcus pentosaceus PR-1 -- probiotic -- characterisation -- probiotic properties; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnac089
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract: Although probiotics have been isolated from different sources, few were isolated from traditional Chinese medicine. The current study firstly isolates Pulsatilla Radix -utilising Pediococcus pentosaceus PR-1 from human faeces. Subsequently, the tolerance of PR-1 to low pH, bile salts, simulated gastric juice and succus entericus, antioxidant activity, antimicrobial activity, cholesterol assimilation and antibiotics susceptibility were investigated. After 2 h of incubation at pH 2.0, over 80% of PR-1 survived. The cell viability of PR-1 at 2 h under 0.1% bile salt condition was 99.2%. The survival rate of PR-1 in gastric juice and succus entericus was 64.48% and 81.86%, respectively. Cell-free supernatant of PR-1 culture also showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Salmonella typhimurium . Besides, antioxidant activity of PR-1 CFS was significantly greater than cell pellet. PR-1 was shown to be resistant to kanamycin, streptomycin, vancomycin and norfloxacin and was able to lower the cholesterol level to 72.5±1.5%. In addition, PR-1 displayed γ-haemolysis and was non-pathogenic. Abstract : Chinese medicine Pulsatilla Radix was applied to screen a potentially probiotic Pediococcus pentosaceus isolate.