Intrinsic chlorine resistance of bacteria modulated by glutaminyl-tRNA biosynthesis in drinking water supply systems. (December 2022)

• Record Type: Journal Article
• Authors: Miao, Xiaocao; Han, Xue; Liu, Chenxu; Bai, Xiaohui
• Is Part Of: Chemosphere. Volume 308:Part 2(2022)
• Publication Date: 2022-12
• Subjects: Chlorine-resistant bacteria -- Glutamate-tRNA ligase -- Drinking water -- Intrinsic molecular mechanism; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.136322
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The existence of chlorine-resistant bacteria (CRB) in drinking water supply systems (DWSSs) results in significant challenges to the biological security of drinking water. However, little is known about the intrinsic chlorine-resistant molecular metabolic mechanism of bacteria in DWSSs. This research explored the microbial interactions and the key metabolic pathways that modulate the chlorine resistance of bacteria in full-scale chloraminated DWSSs. The dominant CRB, including Bdellovibrio, Bradyrhizobium, Peredibacter, Sphingomonas, and Hydrogenophaga, strongly interacted with each other to maintain basic metabolism. A total of 4.21% of the bacterial metabolic pathways were key and specific to chlorine-resistant bacteria. Glutaminyl-tRNA biosynthesis was the dominant metabolic pathway of CRB in the target DWSSs. After chloramine disinfection, the relative abundance of glutamate-tRNA ligase ( GlnRS ) and the related orthologous genes increased by 10.11% and 14.58%, respectively. The inactivation rate of the GlnRS overexpression strain (81.40%) was lower than that of the wild-type strain (90.11%) after exposure to chloramine. Meanwhile, the growth rate of the GlnRS overexpression strain was higher than that of the wild-type strain. Glutaminyl-tRNA biosynthesis can enhance chlorine resistance in DWSSs. Graphical abstract: Image 1 Highlights: The dominant CRB interacted strongly to maintain basic metabolism. Glutaminyl-tRNA biosynthesis was the key metabolic pathway of CRB. The GlnRS overexpression strain showed a lower inactivation rate after chloramination. The chlorine resistance of bacteria can be modulated by GlnRS in DWSSs.