The speciation of arsenic in the muscle tissue of inland and coastal freshwater fish from a remote boreal region. (December 2022)

• Record Type: Journal Article
• Authors: Lescord, Gretchen L.; Johnston, Thomas A.; Ponton, Dominic E.; Amyot, Marc; Lock, Alan; Gunn, John M.
• Is Part Of: Chemosphere. Volume 308:Part 1(2022)
• Publication Date: 2022-12
• Subjects: Arsenic -- Arsenobetaine -- Speciation -- Anadromy -- Freshwater -- Stable isotopes; AsB Arsenobetaine -- DMA Dimethylarsonic acid -- MMA Monomethylarsonic acid -- iAs Inorganic arsenic; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.136140
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Elevated concentrations of total arsenic (As) have been reported in boreal freshwater fish in both human-impacted and relatively pristine areas. We assessed the arsenic speciation profiles in muscle tissue of six fish species (n = 300) sampled from nine locations across a remote freshwater watershed in northern Ontario, Canada, extending from inland headwater lakes to the coastal marine confluence. Of the five arsenic species measured, only arsenobetaine (AsB) and dimethylarsinic acid (DMA) were detected in these fish. Riverine fish had up to 10-fold higher total [As] when compared to lacustrine fish. On average, these riverine fish also had higher percentages of AsB (%AsB, 60 ± 26%) and lower percentages of unmeasured arsenic (%UNM, 20 ± 21%), compared to lacustrine fish (28 ± 18% and 52 ± 21% %AsB and %UNM, respectively). DMA percentages (%DMA) were relatively consistent across the watershed, averaging 20 ± 21% across all fish. We examined ecological drivers of As speciation and found that %AsB increased slightly with fish weight in large-body predatory fish, but not in forage fish or insectivores. Furthermore, %AsB was positively related to trophic elevation (inferred from δ 15 N) in lacustrine fish across 3 out of 4 communities and within some populations. Lastly, riverine fish with a more marine-based diet had markedly higher %AsB when compared to fish with more freshwater-based diets, indicating an effect of anadromy on arsenic speciation. Overall, knowledge on arsenic speciation in freshwater fish has been limited and these results indicate potential drivers that can be considered in future studies. Furthermore, the absence of toxic inorganic As species in these boreal fish is an important consideration for future environmental monitoring practices and risk assessments, some of which assume 10–20% of total [As] in fish is present as toxic inorganic As. Additional studies on As bioaccumulation and biotransformation are needed in freshwater systems, particularly at the base of aquatic food webs. Graphical abstract: Image 1 Highlights: We analyzed 300 wild-caught fish from a remote region for arsenic (As) speciation. No inorganic As, the most harmful forms, was found in any of the fish tested. Arsenobetaine (AsB) was the dominant form of As found. Both fish size and trophic ecology impacted the speciation of arsenic in muscle. Results have implications for monitoring of total arsenic in fish.