Effective plasmid delivery to a plasmid-free Bacillus natto strain by a conjugational transfer system. (1st September 2022)

• Record Type: Journal Article
• Authors: Itaya, Mitsuhiro; Sato, Mitsuru; Watanabe, Satoru; Kataoka, Masakazu
• Is Part Of: Journal of biochemistry. Volume 172:Number 5(2022)
• Page Start: 313
• Page End: 319
• Publication Date: 2022-09-01
• Subjects: genetic engineering -- conjugational transfer -- Bacillus subtilis -- Bacillus natto -- antibiotics; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Electronic journals; 572.05
• DOI: 10.1093/jb/mvac069
• Languages: English
• ISSNs: 0021-924X

Abstract

Abstract: In this study, a Bacillus natto strain named NEST141 was constructed. The strain carries no plasmids and is an authentic proline auxotroph—a feature that confers effective selection conditions for plasmids transferred from a donor, such as Bacillus subtilis 168, via a pLS20-based conjugational transfer system. We have provided a standard effective protocol for the delivery of plasmids larger than 50 kilobase pairs. These results indicate that the B. natto NEST141 strain can become a standard model, like B. subtilis 168, for extensive genetic engineering with diverse applications. Graphical Abstract: