Acuticoccus kalidii sp. nov., a 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing endophyte from a root of Kalidium cuspidatum. Issue 10 (17th October 2022)

• Record Type: Journal Article
• Authors: Tian, Jing; Xu, Lian; Zhang, Xu; Sun, Ji-Quan
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 10(2022)
• Publication Date: 2022-10-17
• Subjects: Acuticoccus -- ACC deaminase -- endophytic -- halophyte -- phylogenetic analysis -- taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005521
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A 1-aminocyclopropane-1-carboxylate (ACC) deaminase-producing, Gram-stain-negative, strictly aerobic, non-motile, yellow-reddish, oval-shaped bacterial strain, designated M5D2P5 T, was isolated from a root of Kalidium cuspidatum, in Tumd Right Banner, Inner Mongolia, PR China. M5D2P5 T grew at 10–40 °C (optimum 30–35 °C), pH 5.0–10.0 (optimum pH 8.0) and with 0–7% NaCl (optimum 3.0 %). The strain was positive for catalase and oxidase. The phylogenetic trees based on 16S rRNA gene sequences indicated that M5D2P5 T clustered with Acuticoccus yangtzensis JL1095 T, and shared 98.0, 97.3, 97.2, 96.9 and less than 96.9 % 16S rRNA gene similarities to A. yangtzensis JL1095 T, Acuticoccus mangrovi B2012 T, Acuticoccus sediminis PTG4-2 T, Acuticoccus kandeliae J103 T, and all the other type strains, respectively. However, the phylogenomic tree showed it clustered with A. kandeliae J103 T . M5D2P5 T contained Q-10 as the major respiratory quinone, as well as two minor respiratory quinones, Q-7 and Q-8. Its major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, an unidentified glycolipid, and four unidentified lipids. The genomic DNA G+C content was 66.5 %. The digital DNA–DNA hybridization score and the average nucleotide identity based on blast values of M5D2P5 T to A. yangtzensis JL1095 T, A. kandeliae J103 T, A. mangrovi B2012 T, and A. sediminis PTG4-2 T, were 20.8, 23.7, 20.7, and 21.5 %, and 73.3, 79.5, 74.4, and 73.7 %, respectively. The phylogenetic and phenotypic characteristics allowed the discrimination of M5D2P5 T from its phylogenetic relatives. The novel species Acuticoccus kalidii sp. nov. is therefore proposed, and the type strain is M5D2P5 T (=CGMCC 1.19149 T =KCTC 92132 T ).