Mechanical cues modulate cellular uptake of nanoparticles in cancer via clathrin-mediated and caveolae-mediated endocytosis pathways. (28th February 2019)

• Record Type: Journal Article
• Authors: Wei, Xiao; Wei, Rui; Jiang, Guiying; Jia, Yijuan; Lou, Hua; Yang, Zongyuan; Luo, Danfeng; Huang, Quanfu; Xu, Sen; Yang, Xin; Zhou, Ying; Li, Xiaoting; Ji, Teng; Hu, Junbo; Xi, Ling; Ma, Ding; Ye, Fei; Gao, Qinglei
• Is Part Of: Nanomedicine. Volume 14:Number 5(2019)
• Page Start: 613
• Page End: 626
• Publication Date: 2019-02-28
• Subjects: cancer -- cellular uptake -- endocytic pathways -- matrix stiffness -- nanoparticle; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2018-0334
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To investigate the influence of tissue mechanics on the cellular uptake efficiency of nanoparticles (NPs) in cancer.Materials & methods: Collagen-coated polyacrylamide gels were prepared as model substrates. Coumarin 6-loaded poly(lactic-co-glycolic) acid micelles (C6-NPs) were prepared to investigate the cellular uptake of NPs.Results: We demonstrated that substrate stiffness modulated the cellular uptake of NPs of cancer. Mechanistically, mechanical cues exerted influence on the clathrin-mediated endocytosis and caveolae-mediated endocytosis pathways, which mediated stiffness-regulated cellular uptake of NPs.Conclusion: Our findings shed light on the regulatory role of the mechanical cues on the cellular uptake of NPs and will facilitate the selection of clinical patients who might benefit from a given nanotherapy.