Magnetic resonance spectroscopy in the rodent brain: Experts' consensus recommendations. (26th August 2020)

• Record Type: Journal Article
• Authors: Lanz, Bernard; Abaei, Alireza; Braissant, Olivier; Choi, In‐Young; Cudalbu, Cristina; Henry, Pierre‐Gilles; Gruetter, Rolf; Kara, Firat; Kantarci, Kejal; Lee, Phil; Lutz, Norbert W.; Marjańska, Małgorzata; Mlynárik, Vladimír; Rasche, Volker; Xin, Lijing; Valette, Julien
• Other Names: Behar Kevin investigator.; Boumezbeur Fawzi investigator.; Deelchand Dinesh Kumar investigator.; Dreher Wolfgang investigator.; Klaunberg Brenda A. investigator.; Ligneul Clemence investigator.; Lindquist Diana M. investigator.; Öz Gülin investigator.; Tkáč Ivan investigator.; Williams Steve R. investigator.; Choi In‐Young guestEditor.; Kreis Roland guestEditor.
• Is Part Of: NMR in biomedicine. Volume 34:Number 5(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-08-26
• Subjects: anesthesia -- brain metabolism -- consensus review -- dynamic MRS -- neurochemical profile -- preclinical MRS; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.4325
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : In vivo MRS is a non‐invasive measurement technique used not only in humans, but also in animal models using high‐field magnets. MRS enables the measurement of metabolite concentrations as well as metabolic rates and their modifications in healthy animals and disease models. Such data open the way to a deeper understanding of the underlying biochemistry, related disturbances and mechanisms taking place during or prior to symptoms and tissue changes. In this work, we focus on the main preclinical 1 H, 31 P and 13 C MRS approaches to study brain metabolism in rodent models, with the aim of providing general experts' consensus recommendations (animal models, anesthesia, data acquisition protocols). An overview of the main practical differences in preclinical compared with clinical MRS studies is presented, as well as the additional biochemical information that can be obtained in animal models in terms of metabolite concentrations and metabolic flux measurements. The properties of high‐field preclinical MRS and the technical limitations are also described. Abstract : We present here the main preclinical 1 H, 31 P and 13 C MRS approaches to study brain metabolism in rodent models, with the aim of providing general experts' consensus recommendations (animal models, anesthesia, data acquisition protocols). An overview of the main practical differences in preclinical compared with clinical MRS studies as well as the additional biochemical information is presented, and the technical limitations are also described.