Differentiation of Aflatoxigenic and Non-Aflatoxigenic Strains of Aspergilli by FT-IR Spectroscopy. Issue 8 (August 2014)

• Record Type: Journal Article
• Authors: Atkinson, Curtis; Pechanova, Olga; Sparks, Darrell L.; Brown, Ashli; Rodriguez, Jose M.
• Is Part Of: Applied spectroscopy. Volume 68:Issue 8(2014)
• Page Start: 920
• Page End: 924
• Publication Date: 2014-08
• Subjects: Aspergillus flavus -- Aspergillus parasiticus -- Fourier transform infrared spectroscopy -- Multivariate analysis -- Differentiation -- Aflatoxigenic -- Non-aflatoxigenic; Spectrum analysis -- Periodicals; 543.505
• DOI: 10.1366/13-06993
• Languages: English
• ISSNs: 0003-7028

Abstract

Fourier transform infrared spectroscopy (FT-IR) is a well-established and widely accepted methodology to identify and differentiate diverse microbial species. In this study, FT-IR was used to differentiate 20 strains of ubiquitous and agronomically important phytopathogens of Aspergillus flavus and Aspergillus parasiticus. By analyzing their spectral profiles via principal component and cluster analysis, differentiation was achieved between the aflatoxin-producing and nonproducing strains of both fungal species. This study thus indicates that FT-IR coupled to multivariate statistics can rapidly differentiate strains of Aspergilli based on their toxigenicity.