Microarray Chip Development Using Infrared Imaging for the Identification of Catfish Species. Issue 12 (December 2014)

• Record Type: Journal Article
• Authors: Handy, Sara M.; Chizhikov, Vladimir; Yakes, Betsy Jean; Paul, Stephen Z.; Deeds, Jonathan R.; Mossoba, Magdi M.
• Is Part Of: Applied spectroscopy. Volume 68:Issue 12(2014)
• Page Start: 1365
• Page End: 1373
• Publication Date: 2014-12
• Subjects: Catfish -- Mid-infrared -- Chemical imaging -- Deoxyribonucleic acid -- DNA microarrays -- Raman spectroscopy -- DNA oligoprobes; Spectrum analysis -- Periodicals; 543.505
• DOI: 10.1366/14-07505
• Languages: English
• ISSNs: 0003-7028

Abstract

Several families of catfish species are extensively aquacultured around the world; however, only those from the family Ictaluridae can be labeled as catfish in the United States. Non-Ictalurid catfish species that are marketed as "catfish" in the USA are considered misbranded. Misbranding in general has led to an increased interest in developing deoxyribonucleic acid (DNA)-based methods such as DNA barcoding, polymerase chain reaction restriction fragment length polymorphism, and DNA microarrays with fluorescence detection for the identification of fish species. In this proof-of-concept study, DNA microarrays coupled with a newly developed mid-infrared imaging detection method were applied to the identification of seven species of catfish for the first time. Species-specific DNA probes targeting three regions per species of the cytochrome c oxidase 1 (barcoding) gene were developed and printed as microarrays on glass slides. Deoxyribonucleic acid targets labeled with biotin were hybridized to their complementary probes using a strategy that allowed the selective formation of a silver layer on hybridized spots needed for detection. Using this three-probe format, the seven species were all identified correctly, even when a limited number of false positive spots were observed. Raman spectroscopy was employed to further characterize the arrays.