Long-Pulsed Luminescence for the Measurement of Dissolved Oxygen. Issue 3 (March 2014)

• Record Type: Journal Article
• Authors: Fitzgerald, Noel S.; Burgess, Lloyd W.; Yang, Jeffrey C.-Y.; Kim, Prince J.; Jang, Sei-Hum; Jen, Alex K.-Y.
• Is Part Of: Applied spectroscopy. Volume 68:Issue 3(2014)
• Page Start: 315
• Page End: 323
• Publication Date: 2014-03
• Subjects: Oxygen measurement -- Long-pulsed luminescence -- LPL -- Phosphorescence -- Exponential decay -- Lifetime measurement -- Rapid lifetime determination -- Platinum porphyrin; Spectrum analysis -- Periodicals; 543.505
• DOI: 10.1366/13-07195
• Languages: English
• ISSNs: 0003-7028

Abstract

Thin-film luminescent sensors were used to measure dissolved oxygen in picoliter volumes for the purpose of monitoring single-cell oxygen consumption rates, and that work served as the motivation for the development of the method described here. A few different platinum porphyrin sensor materials were examined, with all measurements conducted microscopically. By employing convolution theory to understand observed responses, including an unexpected red luminescent emission from an optic, we developed a new, rapid method for the determination of exponential decay lifetime. This new method of long-pulsed luminescence offers substantially improved signal-to-noise ratios for detected signals as long as self-illumination sources are carefully controlled in the experimental set-up.