Size effect of cellulose nanocrystals in cellular internalization and exosome-packaging exocytosis. (15th December 2022)

• Record Type: Journal Article
• Authors: Zhao, Xiaoping; Wang, Qin; Zhu, Ge; Ma, Jingzhi; Lin, Ning
• Is Part Of: Carbohydrate polymers. Volume 298(2022)
• Publication Date: 2022-12-15
• Subjects: Cellulose nanocrystals -- Reducing end modification -- Cellular exocytosis -- Cell internalization; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.120131
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: With the purpose of investigating the bio-interaction between CNC and cells, the end-fluorescence CNC (FCNC) and surface-adsorbed glucosamine [(g)FCNC] were prepared by the region-selective modification and electrostatic adsorption strategy in this study. The cell growth was arrested in a time dependent manner when incubated with CNC in the low glucose environment. The small-size FCNC was preferred by the cells with the observation of higher affinity. Specifically, the affinity of (g)FCNC to the phagocytic cell RAW264.7 cell and kidney derived cell 293 t was generally increased in response to the "Warburg effect", which on the contrary was observed with the weak effect in the tumor cells. We confirmed the exocytosis of internalized rod-like nanocrystal was achieved by the packaging effect of exosomes. Our results revealed the underappreciated bio-interaction between CNC and different cells, which contributed the evidence of an inspiration for this natural nanomaterial in the cell-level application. Graphical abstract: Unlabelled Image Highlights: Six cellulose nanocrystals (CNCs) with the varied scales are prepared by hydrolysis. Bio-interaction analysis of size effect was demonstrated for CNCs with five cells. Cellular internalization of CNCs was investigated under normal and low glucose. Package of CNCs in the exosome was observed for cellular exocytosis behavior.