Purification of Self‐Assembled DNA Tetrahedra Using Gel Electrophoresis. Issue 9 (16th September 2022)

• Record Type: Journal Article
• Authors: Patel, Akul; Valsangkar, Vibhav; Halvorsen, Ken; Chandrasekaran, Arun Richard
• Is Part Of: Current protocols. Volume 2:Issue 9(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-09-16
• Subjects: DNA nanotechnology -- DNA tetrahedra -- gel electrophoresis -- purification -- self‐assembly; Life sciences -- Laboratory manuals -- Periodicals; Biology -- Laboratory manuals -- Periodicals; Life sciences -- Technique -- Periodicals; Biology -- Technique -- Periodicals; 570.028
• DOI: 10.1002/cpz1.560
• Languages: English
• ISSNs: 2691-1299

Abstract

Abstract: DNA nanostructures have found applications in a variety of fields such as biosensing, drug delivery, cellular imaging, and computation. Several of these applications require purification of the DNA nanostructures once they are assembled. Gel electrophoresis–based purification of DNA nanostructures is one of the methods used for this purpose. Here, we describe a step‐by‐step protocol for a gel‐based method to purify self‐assembled DNA tetrahedra. With further optimization, this method could also be adapted for other DNA nanostructures. © 2022 Wiley Periodicals LLC. Basic Protocol : Purification of self‐assembled DNA tetrahedra