Molecular reactivity profiling upon immunotherapy with a 300 IR sublingual house dust mite tablet reveals marked humoral changes towards major allergens. Issue 10 (9th May 2022)

• Record Type: Journal Article
• Authors: Potapova, Ekaterina; Bordas‐Le Floch, Véronique; Schlederer, Thomas; Vrtala, Susanne; Huang, Huey‐Jy; Canonica, Giorgio W.; Valenta, Rudolf; Matricardi, Paolo M.; Mascarell, Laurent
• Is Part Of: Allergy. Volume 77:Issue 10(2022)
• Page Start: 3084
• Page End: 3095
• Publication Date: 2022-05-09
• Subjects: allergen microarray -- allergen‐specific immunotherapy -- house dust mite allergy -- molecular reactivity profile -- SLIT tablet; Allergy -- Periodicals; 616.97
• DOI: 10.1111/all.15327
• Languages: English
• ISSNs: 0105-4538

Abstract

Abstract: Background: Molecular antibody reactivity profiles have not yet been studied in depth in patients treated by sublingual house dust mite (HDM) tablet immunotherapy. Humoral immune responses to a large panel of HDM mite allergens were studied using allergen microarray technology in a subset of clinically defined high and low responder patients from a double‐blind placebo‐controlled allergen‐specific immunotherapy (AIT) trial using sublingual 300 IR HDM tablets. Methods: Serum levels of IgE, IgG and IgG4 to 13 Dermatophagoides pteronyssinus molecules were measured at baseline and after 1‐year AIT, using allergen microarrays in 100 subjects exhibiting high or low clinical benefit. Results: Der p 1, Der p 2 and Der p 23 were the most frequently recognized allergens in the study population. Patients with HDM‐related asthma had significantly higher allergen‐specific IgE levels to Der p 1 and Der p 23. No significant difference in the distribution of allergen sensitization pattern was observed between high and low responders. An increase in serum allergen‐specific IgG and IgG4 occurred upon AIT, in particular to allergens Der p 1, Der p 2 and Der p 23 ( p < 0.0001). Conclusions: We confirm for our study population that Der p 1‐ and Der p 23‐specific IgE levels are associated with asthma. IgE reactivity profiles were not predicitive of sublingual AIT outcomes, with 300 IR tablets as efficacious in pauci‐ and multi‐sensitized subjects. Our study is the first to demonstrate the induction of IgG and IgG4 specific for the HDM allergens Der p 1, Der p 2 and Der p 23 by sublingual AIT. Abstract : This study evaluated humoral responses to HDM allergen panel in a subset of clinically defined high and low responder patients from a DBPC AIT trial using sublingual HDM tablets. Der p 1, Der p 2 and Der p 23 were the most frequently recognized allergens in the study population. Patients with HDM‐related asthma had significantly higher Der p 1‐ and Der p 23‐specific IgE levels compared to non‐asthmatic. Upon AIT, an increase in allergen‐specific IgG and IgG4 was observed, in particular Der p 1‐, Der p 2‐ and Der p 23‐specific.Abbreviations: AIT, allergen immunotherapy; DBPC, double‐blind placebo‐controlled; HDM, house dust mite; Ig, immunoglobulin