Cover Feature: Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement (ChemPhysChem 19/2022). Issue 19 (6th October 2022)

• Record Type: Journal Article
• Authors: Adam, Virgile; Hadjidemetriou, Kyprianos; Jensen, Nickels; Shoeman, Robert L.; Woodhouse, Joyce; Aquila, Andrew; Banneville, Anne‐Sophie; Barends, Thomas R. M.; Bezchastnov, Victor; Boutet, Sébastien; Byrdin, Martin; Cammarata, Marco; Carbajo, Sergio; Eleni Christou, Nina; Coquelle, Nicolas; De la Mora, Eugenio; El Khatib, Mariam; Moreno Chicano, Tadeo; Bruce Doak, R.; Fieschi, Franck; Foucar, Lutz; Glushonkov, Oleksandr; Gorel, Alexander; Grünbein, Marie Luise; Hilpert, Mario; Hunter, Mark; Kloos, Marco; Koglin, Jason E.; Lane, Thomas J.; Liang, Mengning; Mantovanelli, Angela; Nass, Karol; Nass Kovacs, Gabriela; Owada, Shigeki; Roome, Christopher M.; Schirò, Giorgio; Seaberg, Matthew; Stricker, Miriam; Thépaut, Michel; Tono, Kensuke; Ueda, Kiyoshi; Uriarte, Lucas M.; You, Daehyun; Zala, Ninon; Domratcheva, Tatiana; Jakobs, Stefan; Sliwa, Michel; Schlichting, Ilme; Colletier, Jacques‐Philippe; Bourgeois, Dominique; Weik, Martin;
• Is Part Of: Chemphyschem. Volume 23:Issue 19(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-10-06
• Subjects: nanoscopy -- photoswitchable fluorescent proteins -- serial femtosecond crystallography -- switching contrast -- quantum chemistry; Chemistry, Physical and theoretical -- Periodicals; 541.05
• DOI: 10.1002/cphc.202200693
• Languages: English
• ISSNs: 1439-4235

Abstract

Abstract : The Cover Feature displays a comparison of chromophore conformations in the X‐ray crystal structures of the reversibly photoswitchable fluorescent protein rsEGFP2 in its on state (green) and its V151L (purple) and V151A (yellow) variants in their off states. The latter displays an increased photoswitching contrast, as indicated by the displayed switching kinetics. Cover design by Virgile ADAM. More information can be found in the Research Article by Dominique Bourgeois, Martin Weik and co‐workers .