IP & OEIP – Cytokinin Micro Application Modulates Root Development with High Spatial Resolution. Issue 10 (28th April 2022)

• Record Type: Journal Article
• Authors: Pařízková, Barbora; Antoniadi, Ioanna; Poxson, David J.; Karady, Michal; Simon, Daniel T.; Zatloukal, Marek; Strnad, Miroslav; Doležal, Karel; Novák, Ondřej; Ljung, Karin
• Is Part Of: Advanced materials technologies. Volume 7:Issue 10(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-28
• Subjects: arabidopsis -- cytokinin -- organic bioelectronics -- hormone delivery -- lateral root -- root development -- spatial resolution; Materials science -- Periodicals; Technological innovations -- Periodicals; Materials science; Technological innovations; Periodicals; 620.1105
• DOI: 10.1002/admt.202101664
• Languages: English
• ISSNs: 2365-709X

Abstract

Abstract: State‐of‐the‐art technology based on organic electronics can be used as a flow‐free delivery method for organic substances with high spatial resolution. Such highly targeted drug micro applications can be used in plant research for the regulation of physiological processes on tissue and cellular levels. Here, for the first time, an organic electronic ion pump (OEIP) is reported that can transport an isoprenoid‐type cytokinin, N 6 ‐isopentenyladenine (iP), to intact plants. Cytokinins (CKs) are plant hormones involved in many essential physiological processes, including primary root (PR) and lateral root (LR) development. Using the Arabidopsis thaliana root as a model system, efficient iP delivery is demonstrated with a biological output – cytokinin‐related PR and LR growth inhibition. The spatial resolution of iP delivery, defined for the first time for an organic compound, is shown to be less than 1 mm, exclusively affecting the OEIP‐targeted LR. Results from the application of the high‐resolution OIEP treatment method confirm previously published findings showing that the influence of CKs may vary at different stages of LR development. Thus, OEIP‐based technologies offer a novel, electronically controlled method for phytohormone delivery that could contribute to unraveling cytokinin functions during different developmental processes with high specificity. Abstract : The organic electronic ion pump (OEIP) represents a state‐of‐the‐art device for drug delivery that provides great spatial and temporal resolution of compound distribution. Here, the transport parameters for efficient delivery of plant hormone cytokinin—isopentenyladenine (iP)—are optimized. OEIP‐targeted iP treatment modulates cytokinin root responses with a resolution of >1 mm.