Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19. Issue 10 (23rd August 2022)

• Record Type: Journal Article
• Authors: Kellogg, Riss M.; Moran, Dawn M.; McIlvin, Matthew R.; Subhas, Adam V.; Allen, Andrew E.; Saito, Mak A.
• Is Part Of: Limnology and oceanography. Volume 67:Issue 10(2022)
• Page Start: 2265
• Page End: 2280
• Publication Date: 2022-08-23
• Subjects: Limnology -- Periodicals; Oceanography -- Periodicals; Océanographie; Limnologie; Limnology; Oceanography; Computer network resources; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); Periodicals; 551.4805
• DOI: 10.1002/lno.12201
• Languages: English
• ISSNs: 0024-3590

Abstract

Abstract: Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaetoceros neogracile RS19, originally isolated from the Ross Sea, Antarctica, has a Zn requirement that cannot be met by Co and thus does not demonstrate a Zn/Co substitution ability as assessed by growth rate. We investigated this diatom's inability to use Co to alleviate Zn‐limited growth rates at the transporter, sensor/chaperone, and metalloenzyme level using metal quota and proteomic analyses of cultures grown over a range of Zn and Co availability. Analysis of total cellular metal quotas revealed that, although incapable of substitution, this diatom still actively assimilated dissolved Co. We furthermore observed distinct trends in the abundance levels of putative α and θ‐CAs, ZIP transporters, Zn fingers, and a Zn chaperone in response to increasing media Zn 2+ . Overall, Co appears to be transported into the cell, but not efficiently utilized by Zn metalloenzymes.