Normal mesenchymal stem cells can improve the abnormal function of T cells in psoriasis via upregulating transforming growth factor‐β receptor. Issue 10 (29th June 2022)

• Record Type: Journal Article
• Authors: Jiao, Juanjuan; Zhao, Xincheng; Wang, Ying; Liang, Nannan; Li, Juan; Yang, Xiaohong; Xing, Jianxiao; Zhou, Ling; Li, Junqin; Hou, Ruixia; Li, Xinhua; Zhang, Kaiming
• Is Part Of: Journal of dermatology. Volume 49:Issue 10(2022)
• Page Start: 988
• Page End: 997
• Publication Date: 2022-06-29
• Subjects: CD3+ T cells -- dermal mesenchymal stromal cells -- immunosuppressive -- psoriasis -- transforming growth factor‐β receptors; Dermatology -- Periodicals; Dermatology -- Japan -- Periodicals; Skin -- Diseases -- Periodicals; 616.5005
• DOI: 10.1111/1346-8138.16490
• Languages: English
• ISSNs: 0385-2407

Abstract

Abstract: Psoriasis, a chronic inflammatory skin disease, is a refractory disorder. Previous studies have shown that the imbalance of the T‐helper (Th)17/regulatory T cells (Treg) results in the immune imbalance of T cells in psoriatic patients, and that mesenchymal stem cells display an immunosuppressive role by promoting the differentiation of T cells into Treg, leading to a reduction in the proportion of Th17/Treg. Utility of mesenchymal stem cells is becoming a new approach for the treatment of immune disorders. Following co‐culture of dermal mesenchymal stromal cells (DMSC) and CD3 + T cells with or without transforming growth factor (TGF)‐β receptor inhibitor, the biological function and relative signal pathway of CD3 + T cells were assessed by flow cytometry, transwell, real‐time polymerase chain reaction and western blotting, respectively. Normal DMSC were more potent than psoriatic DMSC in inhibition of CD3 + T‐cell proliferation, and stimulation of CD3 + T‐cell apoptosis than psoriasis DMSC. Moreover, normal DMSC decreased the ratio of Th17/Treg, while enhancing the immunosuppressive effect of Tregs on effector T cells. However, TGF‐β receptor (TGF‐βR) inhibitor attenuated the effect of normal DMSC on CD3 + T cells and Th17/Treg ratio. Additionally, the normal DMSC were more potent than the psoriatic DMSC in increasing TGF‐β receptors and activation of TGF‐β/SMAD pathway in psoriatic CD3 + T cells. In conclusion, normal DMSC can partially improve the biological function and immunosuppressive ability of psoriatic CD3 + T cells, possibly via upregulating the TGF‐β receptors.