Imputation of the major histocompatibility complex region identifies major independent variants associated with bullous pemphigoid and dermatomyositis in Han Chinese. Issue 10 (25th June 2022)

• Record Type: Journal Article
• Authors: Fan, Wencheng; Li, Zhuo; Wang, Yirui; Zhang, Chang; Liu, Hao; Wang, Daiyue; Bai, Yuanming; Luo, Sihan; Li, Yuanyuan; Qin, Qin; Chen, Weiwei; Yong, Liang; Zhen, Qi; Yu, Yafen; Ge, Huiyao; Mao, Yiwen; Cao, Lu; Zhang, Ruixue; Hu, Xia; Yu, Yanxia; Li, Bao; Sun, Liangdan
• Is Part Of: Journal of dermatology. Volume 49:Issue 10(2022)
• Page Start: 998
• Page End: 1004
• Publication Date: 2022-06-25
• Subjects: bullous pemphigoid -- dermatomyositis -- imputation -- major histocompatibility complex -- stepwise regression analysis; Dermatology -- Periodicals; Dermatology -- Japan -- Periodicals; Skin -- Diseases -- Periodicals; 616.5005
• DOI: 10.1111/1346-8138.16499
• Languages: English
• ISSNs: 0385-2407

Abstract

Abstract: As autoimmune skin diseases, both bullous pemphigoid (BP) and dermatomyositis (DM) show significant associations with the major histocompatibility complex (MHC) region. In fact, the coexistence of BP and DM has been previously reported. Therefore, we hypothesized that there may be a potential genetic correlation between BP and DM. Based on data for 312 BP patients, 128 DM patients, and 6793 healthy control subjects, in the MHC region, we imputed single‐nucleotide polymorphisms (SNP), insertions and deletions (INDEL), and copy number variations (CNV) using the 1KGP phase 3 dataset and amino acids (AA) and SNP using a Han‐MHC reference database. An association study revealed the most significant SNP associated with BP, namely, rs580921 ( p = 1.06E‐08, odds ratio [OR] = 1.61, 95% confidence interval [CI] = 1.37–1.90), which is located in the C6orf10 gene, and the most significant classic human leukocyte antigen (HLA) allele associated with DM, namely, HLA‐DPB1*1701 ( p = 6.56E‐10, OR = 3.61, 95% CI = 2.40–5.42). Further stepwise regression analyses with rs580921 identified a threonine at position 163 of the HLA‐B gene as a new independent disease‐associated AA, and HLA‐DPB1*1701 indicated that no loci were significant. Three‐dimensional ribbon models revealed that the HLA‐B AA position 163 ( p = 3.93E‐07, OR = 1.64, 95% CI = 1.35–1.98) located in the α2 domain of the HLA‐B molecule was involved in the process of specific antigen presentation. The calculations showed that there was no significant genetic correlation between BP and DM. Our study identified three significant loci in the MHC region, proving that the HLA region was significantly correlated with BP and DM separately. Our research highlights the key role of the MHC region in disease susceptibility.