Comparison of the effects of platelet concentrates produced by high and low-speed centrifugation protocols on the healing of critical-size defects in rat calvaria: a microtomographic and histomorphometric study. (17th November 2022)

• Record Type: Journal Article
• Authors: da Silva, Lucia Moitrel Pequeno; Sávio, Débora de Souza Ferreira; de Ávila, Felipe Correa; Vicente, Raphael Martini; Reis, Gabriel Guerra David; Denardi, Ricardo Junior; da Costa, Natacha Malu Miranda; Silva, Pedro Henrique Felix; Mourão, Carlos Fernando de Almeida Barros; Miron, Richard J.; Messora, Michel Reis
• Is Part Of: Platelets. Volume 33:Number 8(2022)
• Page Start: 1175
• Page End: 1184
• Publication Date: 2022-11-17
• Subjects: Blood platelet -- bone regeneration -- fibrin -- platelet-rich fibrin; Blood platelets -- Periodicals; Blood Platelets -- Periodicals; 615.39
• DOI: 10.1080/09537104.2022.2071851
• Languages: English
• ISSNs: 0953-7104

Abstract

Abstract: The current study evaluated the healing of critical-size defects (CSD) created in rat calvaria treated with platelet concentrates produced by high-speed (Leukocyte- and Platelet-Rich Fibrin - L-PRF) and low-speed (Advanced Platelet-Rich Fibrin - A-PRF) protocols of centrifugation. Twenty-four rats were distributed into three groups: Control, L-PRF, and A-PRF. Five mm diameter CSD were created on the animals' calvaria. The defects of the L-PRF and A-PRF groups were filled with 0.01 ml of L-PRF and A-PRF, respectively. The control group defects were filled with a blood clot only. All animals were euthanized on the 35th postoperative day. Histomorphometric and microtomographic analyses were then performed. The L-PRF and A-PRF groups had significantly higher bone volume and neoformed bone area than those of the control group and lowered bone porosity values (p < .05). No significant differences were observed between A-PRF and L-PRF groups for the analyzed parameters. Therefore, it can be concluded that i) L-PRF and A-PRF potentiated the healing of CSD in rat calvaria; ii) high and low-speed centrifugation protocols did not produce PRF matrices with different biological impacts on the amount of bone neoformation.