A Comparative Investigation of Volatile Organic Compounds of Cattle Rumen Metabolites using HS-SPME and PoraPak-Q Odor Trapping Methods. Issue 4 (4th July 2022)

• Record Type: Journal Article
• Authors: Omondi, Victor O; Bosire, Geoffrey O; Onyari, John M; Getahun, Merid N
• Is Part Of: Analytical chemistry letters. Volume 12:Issue 4(2022)
• Page Start: 451
• Page End: 459
• Publication Date: 2022-07-04
• Subjects: Gas chromatography-Mass spectrometry -- Headspace Solid Phase Microextraction -- PoraPak-Q adsorbent -- Volatile odor compounds; Chemistry, Analytic -- Periodicals; 543.05
• DOI: 10.1080/22297928.2022.2100276
• Languages: English
• ISSNs: 2229-7928

Abstract

Abstract: In the rapidly increasing field of metabolomic research, fast and accurate trapping of volatile odor compounds from biological samples is critical. Here a comparative evaluation of HS-SPME and PoraPak-Q adsorbent odor trapping methods followed by GC-MS analysis were used to determine volatile compounds in cattle rumen. Compared to the PoraPak-Q adsorbent, the HS-SPME method was more effective in trapping diverse metabolites including low molecular weight and highly volatile compounds such as carbon dioxide, acetic and butyric acid. Additionally, using the HS-SPME method, shorter trapping times were achieved (30 minutes) whilst the PoraPak-Q adsorbent required longer time extending beyond 1 hour for effective volatile trapping. In the context of metabolomics analysis from biological samples, the two different methods vary in determination of chemo-diversities, qualitatively and abundance of shared odor, and time required to trap odors, which are critical in such studies. GRAPHICAL ABSTRACT: UF0001