Integrated biosensors for monitoring microphysiological systems. Issue 20 (8th September 2022)

• Record Type: Journal Article
• Authors: Mou, Lei; Mandal, Kalpana; Mecwan, Marvin Magan; Hernandez, Ana Lopez; Maity, Surjendu; Sharma, Saurabh; Herculano, Rondinelli Donizetti; Kawakita, Satoru; Jucaud, Vadim; Dokmeci, Mehmet Remzi; Khademhosseini, Ali
• Is Part Of: Lab on a chip. Volume 22:Issue 20(2022)
• Page Start: 3801
• Page End: 3816
• Publication Date: 2022-09-08
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d2lc00262k
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : This review discusses the state-of-the-art integrated biosensors by providing specific examples, detailing their main advantages in monitoring MPSs, and describing the remaining challenges and potential future developments. Abstract : Microphysiological systems (MPSs), also known as organ-on-a-chip models, aim to recapitulate the functional components of human tissues or organs in vitro . Over the last decade, with the advances in biomaterials, 3D bioprinting, and microfluidics, numerous MPSs have emerged with applications to study diseased and healthy tissue models. Various organs have been modeled using MPS technology, such as the heart, liver, lung, and blood–brain barrier. An important aspect of in vitro modeling is the accurate phenotypical and functional characterization of the modeled organ. However, most conventional characterization methods are invasive and destructive and do not allow continuous monitoring of the cells in culture. On the other hand, microfluidic biosensors enable in-line, real-time sensing of target molecules with an excellent limit of detection and in a non-invasive manner, thereby effectively overcoming the limitation of the traditional techniques. Consequently, microfluidic biosensors have been increasingly integrated into MPSs and used for in-line target detection. This review discusses the state-of-the-art microfluidic biosensors by providing specific examples, detailing their main advantages in monitoring MPSs, and highlighting current developments in this field. Finally, we describe the remaining challenges and potential future developments to advance the current state-of-the-art in integrated microfluidic biosensors.