Company matters: The presence of other genotypes alters traits and intraspecific selection in an Arctic diatom under climate change. (2nd July 2019)

• Record Type: Journal Article
• Authors: Wolf, Klara K. E.; Romanelli, Elisa; Rost, Björn; John, Uwe; Collins, Sinead; Weigand, Hannah; Hoppe, Clara J. M.
• Is Part Of: Global change biology. Volume 25:Number 9(2019)
• Page Start: 2869
• Page End: 2884
• Publication Date: 2019-07-02
• Subjects: allele‐specific qPCR -- artificial population -- genotypic interactions -- intraspecific diversity -- multiple stressors -- ocean acidification -- phenotypic plasticity -- selection dynamics -- strain sorting -- warming; Climatic changes -- Environmental aspects -- Periodicals; Troposphere -- Environmental aspects -- Periodicals; Biodiversity conservation -- Periodicals; Eutrophication -- Periodicals; 551.5
• DOI: 10.1111/gcb.14675
• Languages: English
• ISSNs: 1354-1013

Abstract

Abstract: Arctic phytoplankton and their response to future conditions shape one of the most rapidly changing ecosystems on the planet. We tested how much the phenotypic responses of strains from the same Arctic diatom population diverge and whether the physiology and intraspecific composition of multistrain populations differs from expectations based on single strain traits. To this end, we conducted incubation experiments with the diatom Thalassiosira hyalina under present‐day and future temperature and pCO2 treatments. Six fresh isolates from the same Svalbard population were incubated as mono‐ and multistrain cultures. For the first time, we were able to closely follow intraspecific selection within an artificial population using microsatellites and allele‐specific quantitative PCR. Our results showed not only that there is substantial variation in how strains of the same species cope with the tested environments but also that changes in genotype composition, production rates, and cellular quotas in the multistrain cultures are not predictable from monoculture performance. Nevertheless, the physiological responses as well as strain composition of the artificial populations were highly reproducible within each environment. Interestingly, we only detected significant strain sorting in those populations exposed to the future treatment. This study illustrates that the genetic composition of populations can change on very short timescales through selection from the intraspecific standing stock, indicating the potential for rapid population level adaptation to climate change. We further show that individuals adjust their phenotype not only in response to their physicochemical but also to their biological surroundings. Such intraspecific interactions need to be understood in order to realistically predict ecosystem responses to global change. Abstract : Six freshly isolated strains of the Arctic diatom Thalassiosira hyalina were incubated as mono‐ and multistrain cultures under different temperature and CO2 conditions. Although strains originated from the same water sample, monocultures showed large physiological diversity. When tested all together in multistrain cultures, selection dynamics as well as bulk physiology within these artificial populations differed fundamentally between the two treatments and diverged strongly from predictions based on monoculture traits. This suggests that cells change their phenotype depending on their biological surroundings and that such intraspecific interactions need to be better understood to predict future phytoplankton ecology from experimental data.