A fungal nonribosomal peptide-polyketide hybrid synthase synthesizes 2-pyrrolidinone alkaloid. (22nd October 2022)

• Record Type: Journal Article
• Authors: Rao, Li; Shi, Hu-Cheng; Zou, Yi
• Is Part Of: Tetrahedron. Volume 125(2022)
• Publication Date: 2022-10-22
• Subjects: Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2022.133060
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: A new fungal nonribosomal peptide-polyketide hybrid synthase, namely CogA, was discovered by genome mining from fungus Cordyceps gunnii . Its ability to synthesize 4-hydroxyl 2-pyrrolidinone alkaloid (1 ) via nonenzyme-catalyzed N-1-C-2 closure was confirmed by heterologous expression of it in Aspergillus nidulans . Further engineered biosynthesis of the fluoro-substituted 2-pyrrolidinone alkaloid analogues (4 and 5 ) has also been achieved in vivo .