Altered active pyrene degraders in biosurfactant-assisted bioaugmentation as revealed by RNA stable isotope probing. (15th November 2022)

• Record Type: Journal Article
• Authors: Teng, Tingting; Liang, Jidong; Zhu, Jinwei; Jin, Pengkang; Zhang, Dayi
• Is Part Of: Environmental pollution. Volume 313(2022)
• Publication Date: 2022-11-15
• Subjects: Bioaugmentation -- Biosurfactant -- Natural attenuation -- Pyrene-degrading bacteria -- RNA-SIP; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.120192
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Bioaugmentation is an effective approach for removing pyrene from contaminated sites, and its performance is enhanced by a biosurfactant. To reveal the mechanisms of biosurfactant-assisted bioaugmentation, we introduced RNA stable isotope probing (RNA-SIP) in the pyrene-contaminated soils and explored the impacts of rhamnolipid on the pyrene degradation process. After 12-day degradation, residual pyrene was the lowest in the bioaugmentation treatment (7.76 ± 1.57%), followed by biosurfactant-assisted bioaugmentation (9.86 ± 2.58%) and enhanced natural attenuation (23.97 ± 1.05%). Thirteen well-known and two novel pyrene-degrading bacteria were confirmed to participate in the pyrene degradation. Pyrene degradation was accelerated in the biosurfactant-assisted bioaugmentation, manifested by the high diversity of active pyrene degraders. Our findings expand the knowledge on pyrene degrading bacteria and the mechanisms of pyrene degradation in a bioaugmentation process. Graphical abstract: Image 1 Highlights: Bioaugmentation (BA) and biosurfactant-assisted bioaugmentation (BSBA) accelerated pyrene degradation. 3, 4 and 8 pyrene degraders were identified in enhanced natural attenuation, BA and BSBA. Two novel pyrene degraders were firstly unraveled by RNA-SIP. Exogenous degraders exhibited negative correlations with other soil bacteria. Pyrene-degrading communities were reconstructed in the presence of rhamnolipid.