Identification and quantification of eight alkaloids in Aconitum heterophyllum using UHPLC‐DAD‐QTOF‐IMS: A valuable tool for quality control. Issue 7 (6th July 2022)

• Record Type: Journal Article
• Authors: Punia, Ashwani; Joshi, Robin; Kumar, Rajiv
• Is Part Of: Phytochemical analysis. Volume 33:Issue 7(2022)
• Page Start: 1121
• Page End: 1134
• Publication Date: 2022-07-06
• Subjects: Aconitum -- alkaloids -- ion mobility mass spectrometry -- UHPLC; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.3164
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: Aconitum spp. are prime medicinal plants rich in alkaloids and have been used as the main constituents of traditional medicine in India and China. The whole plant can be toxic and creates pathophysiological conditions inside the human body. Therefore, simultaneous quantification of alkaloids within plant parts and herbal medicines associated with this genus is essential for quality control. Objective: We aimed to develop and validate methods using ultra‐high‐performance liquid chromatography–diode array detector–quadrupole time‐of‐flight ion mobility mass spectrometry (UHPLC‐DAD‐QTOF‐IMS) and to develop an analytical strategy for the identification and quantification of alkaloid compounds (aconitine, hypaconitine, mesaconitine, aconine, benzoylmesaconitine, benzoylaconine, bulleyaconitine A, and deoxyaconitine) from Aconitum heterophyllum . Methodology: We developed a simultaneous identification and quantification method for eight alkaloids using UHPLC‐DAD‐QTOF‐IMS. The method was validated as per International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines and also in IMS mode. Results: The developed method has good linearity (r 2 = 0.997–0.999), LOD (0.63–8.31 μg/mL), LOQ (0.63–2.80 μg/mL), recovery (86.01–104.33%), reproducibility, intra‐ and inter‐day variability (<3.25%), and stability. Significant qualitative and quantitative variations were found among different plant parts (flower, leaf, stem, root, and tuber) and five market products of A. heterophyllum . Furthermore, a total of 21 metabolites were also profiled based on the fragmentation pattern of MS 2 using the validated method. Conclusion: An appropriate mobile phase using acetonitrile and water in a gradient elution gave a satisfactory chromatographic separation of eight Aconitum alkaloids with their adjacent peaks. Therefore, this method could provide a scientific and technical platform for quality control assurance. Abstract : Aconitum heterophyllum Wall ex Royle is an endangered and nontoxic medicinal plant having wide therapeutic effects and used commonly in Indian and Chinese herbal medicine formulation. Here, we developed and validated a method for simultaneous identification and quantification of eight alkaloids from A. heterophyllum using UHPLC‐QTOF‐IMS. Furthermore, a total of 21 metabolites were also profiled based on the fragmentation pattern of MS2 using the validated method. The developed method could provide scientific and technical platform for quality control assurance.