Transfer RNA‐derived fragment 5′tRF‐Gly promotes the development of hepatocellular carcinoma by direct targeting of carcinoembryonic antigen‐related cell adhesion molecule 1. Issue 10 (16th August 2022)

• Record Type: Journal Article
• Authors: Liu, Dekai; Wu, Chengdong; Wang, Jingjie; Zhang, Lufei; Sun, Zhongquan; Chen, Shihong; Ding, Yuan; Wang, Weilin
• Is Part Of: Cancer science. Volume 113:Issue 10(2022)
• Page Start: 3476
• Page End: 3488
• Publication Date: 2022-08-16
• Subjects: 5′tRF‐Gly -- biomarker -- CEACAM1 -- hepatocellular carcinoma -- tRNA‐derived small RNA; Cancer -- Periodicals; Neoplasms -- Periodicals; Research -- Periodicals; Electronic journals; 616.994005
• DOI: 10.1111/cas.15505
• Languages: English
• ISSNs: 1347-9032

Abstract

Abstract: Transfer RNA‐derived fragments are a group of small noncoding single‐stranded RNA that play essential roles in multiple diseases. However, their biological functions in carcinogenesis are not well understood. In this study, 5′tRF‐Gly was found to have significantly high expression in hepatocellular carcinoma (HCC), and the upregulation of 5′tRF‐Gly was positively correlated with tumor size and tumor metastasis. Overexpression of 5′tRF‐Gly induced increased growth rate and metastasis in HCC cells in vitro and in nude mice, while knockdown showed the opposite effect. Carcinoembryonic antigen‐related cell adhesion molecule 1 (CEACAM1) was confirmed to be a direct target of 5′tRF‐Gly in HCC. In addition, the cytological effect of CEACAM1 knockdown proved to be similar to the overexpression of 5′tRF‐Gly. Moreover, attenuation of CEACAM1 expression rescued the 5′tRF‐Gly‐mediated promoting effects on HCC cells. These data show that 5′tRF‐Gly is a new tumor‐promoting factor and could be a potential diagnostic biomarker or new therapeutic target for HCC. Abstract : In the present study, the small RNA high‐throughput sequencing results showed that 5'tRF‐Gly was up‐regulated in HCC tissues and was related to poor prognosis. We demonstrated the oncogenic role of 5'tRF‐Gly and its underlying molecular mechanism in HCC. Furthermore, we first verified CEA cell adhesion molecule 1 (CEACAM1) as the direct target of 5'tRF‐Gly and revealed how CEACAM1 inhibits hepatocellular carcinoma growth and metastasis. Our findings not only elucidate the role of 5'tRF‐Gly and CEACAM1 in HCC but also provide new targets for the prognostic evaluation and treatment of HCC.