Identification and integrated analysis of key differentially expressed circular RNAs in ER-positive subtype breast cancer. (12th November 2018)

• Record Type: Journal Article
• Authors: Yuan, Chenwei; Zhou, Liheng; Zhang, Lei; Yin, Kai; Peng, Jing; Sha, Rui; Zhang, Shan; Xu, Yaqian; Sheng, Xiaonan; Wang, Yaohui; Lin, Yanping; Xu, Shuguang; Yin, Wenjin; Lu, Jinsong
• Is Part Of: Epigenomics. Volume 11:Number 3(2019)
• Page Start: 297
• Page End: 321
• Publication Date: 2018-11-12
• Subjects: bioinformatics -- breast cancer -- circular RNA -- hsa_circ_0087378 -- microarray -- miRNA -- miR-1260b -- noncoding RNA -- SFRP1 -- tumorigenesis; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2018-0147
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To systematically profile and characterize the circular RNA (circRNA) expression pattern in estrogen receptor (ER)-positive breast cancer (BC).Materials & methods: CircRNA expression profile was performed in ER-positive BC and adjacent nontumor tissues. The differentially expressed circRNAs (DECs) was analyzed by bioinformatics. The analysis findings were validated by quantitative real-time PCR.Results: In total, 3653 DECs were detected in our ER-positive BC compared with the control. Bioinformatics analysis showed that some pathways related to cancer, especially BC, were significantly enriched. Additionally, hsa_circ_0087378 was validated to be downregulated in ER-positive BC and the hsa_circ_0087378-miR-1260b-SFRP1 axis was proposed to be a key regulatory pathway.Conclusion: This study revealed the general expression characteristics of specific DECs in ER-positive BC and hsa_circ_0087378 might be a promising candidate target.