Circular RNA circRHOT1 is upregulated and promotes cell proliferation and invasion in pancreatic cancer. (16th November 2018)

• Record Type: Journal Article
• Authors: Qu, Shibin; Hao, Xiaokun; Song, Wenjie; Niu, Kunwei; Yang, Xisheng; Zhang, Xuan; Shang, Runze; Wang, Quancheng; Li, Haimin; Liu, Zhengcai
• Is Part Of: Epigenomics. Volume 11:Number 1(2019)
• Page Start: 1683
• Page End: 1693
• Publication Date: 2018-11-16
• Subjects: ceRNA -- circRHOT1 -- circular RNA -- miRNA -- pancreatic cancer; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2018-0051
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: We aimed to identify the roles of circRHOT1 in pancreatic cancer.Materials & methods: The circRHOT1 was acquired from our previous study followed by quantitative real-time PCR and fluorescence in situ hybridization validation in pancreatic cancer. We used siRNA and shRNA to explore the function of circRHOT1 in pancreatic cancer cells. Bioinformatic analyses were applied to study the potential mechanism of circRHOT1.Results: The circRHOT1 was upregulated in pancreatic cancer and predominantly located in the cytoplasm. Reducing the circRHOT1 expression may inhibit the pancreatic cancer cell proliferation, invasion and migration. The circRHOT1 may play a role in pancreatic cancer through binding miR-26b, miR-125a, miR-330 and miR-382 to regulate multiple tumor-associated pathways.Conclusion: This study demonstrated that circRHOT1 may serve as an oncogenic circRNA that promotes tumor progression.