DNA Methylation and mRNA Expression of B7-H3 Gene in Ankylosing Spondylitis: A Case-Control Study. (3rd October 2022)

• Record Type: Journal Article
• Authors: Chen, Yuting; Wu, Ye; Yang, Hui; Wang, Jinian; Kong, Jiangping; Yu, Lingxiang; Ni, Man; Deng, Yujie; Xu, Shengqian; Yu, Haiyang; Shuai, Zongwen; Pan, Faming
• Is Part Of: Immunological investigations. Volume 51:Number 7(2022)
• Page Start: 2025
• Page End: 2034
• Publication Date: 2022-10-03
• Subjects: Ankylosing spondylitis -- B7-H3 -- DNA methylation -- epigenetics -- mRNA; Immunology -- Periodicals; Immunochemistry -- Periodicals; Cellular immunity -- Periodicals; Communicable diseases -- Periodicals; 616.079
• DOI: 10.1080/08820139.2022.2095285
• Languages: English
• ISSNs: 0882-0139

Abstract

ABSTRACT: Background: Ankylosing spondylitis (AS) is a common inflammatory arthritis, with a high prevalence in patients in their mid-20s. Its pathogenesis is not well understood; however, genetic factors likely play a critical role. Epigenetic DNA changes may be involved in the pathogenesis of AS. In this study, we explored the methylation and transcription levels of the B7-H3 gene and its association with AS in an eastern Chinese Han population. Methods: Peripheral blood of AS patients and healthy controls was used to extract genomic DNA and B7-H3 methylation levels were analyzed using sodium bisulfite followed by multiplex polymerase chain reaction. SPSS software was used to determine the statistical significance of the results. Results: Hypomethylation of the promoter of the B7-H3 gene was observed in AS patients, whereas the B7-H3 gene expression was significantly enhanced in AS patients. Conclusion: Epigenetic modifications of B7-H3 were associated with susceptibility to AS. Hypomethylation of the B7-H3 promoter, which leads to B7-H3 overexpression, may be involved in the pathogenesis of AS.